Literature summary for 2.5.1.20 extracted from

Scott, D.J.; da Costa, B.M.; Espy, S.C.; Keasling, J.D.; Cornish, K.
Activation and inhibition of rubber transferases by metal cofactors and pyrophosphate substrates (2003), Phytochemistry, 64, 123-134.

Search for more literature for 2.5.1.20

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	4 mM, no residual activity	Ficus elastica
EDTA	4 mM, about 10% residual activity	Hevea brasiliensis
EDTA	4 mM, about 20% residual activity	Parthenium argentatum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.04	-	farnesyl diphosphate	pH 7.5, 25°C	Parthenium argentatum
0.4	-	isopentenyl diphosphate	pH 7.5, 25°C	Parthenium argentatum
0.5	-	isopentenyl diphosphate	pH 7.5, 25°C	Hevea brasiliensis
0.8	-	farnesyl diphosphate	pH 7.5, 25°C	Ficus elastica
2.2	-	farnesyl diphosphate	pH 7.5, 25°C	Hevea brasiliensis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	at 10 mM, maximum of activity	Hevea brasiliensis
Mg2+	at 100 mM, maximum of activity	Ficus elastica
Mg2+	at 8 mM, maximum of activity	Parthenium argentatum
Mn2+	at 11 mM, maximum of activity	Ficus elastica
Mn2+	at 3.8 mM, maximum of activity	Hevea brasiliensis
Mn2+	at 6.8 mM, maximum of activity	Parthenium argentatum

Organism

Organism	UniProt	Comment	Textmining
Ficus elastica	-	-	-
Hevea brasiliensis	-	-	-
Parthenium argentatum	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
isopentenyl diphosphate + farnesyl diphosphate	-	Hevea brasiliensis	diphosphate + ?	-	?
isopentenyl diphosphate + farnesyl diphosphate	-	Ficus elastica	diphosphate + ?	-	?
isopentenyl diphosphate + farnesyl diphosphate	-	Parthenium argentatum	diphosphate + ?	-	?

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Release 2023.1 (January 2023)