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Literature summary for 2.5.1.20 extracted from
- Isopentenyl pyrophosphate cis-1,4-polyisoprenyl transferase from guayule (Parthernium argentatum gray) (1984), Plant Physiol., 75, 908-913.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | required for maximal activity | Ficus elastica |  |
| glutathione | required for maximal activity | Parthenium argentatum | |
| glutathione | required for maximal activity | Parthenium hysterophorus | |
| glutathione | required for maximal activity | Ficus lyrata | |
| glutathione | required for maximal activity | Ficus benjamina | |
| glutathione | required for maximal activity | Ficus deltoidea | |
| glutathione | required for maximal activity | Ficus decora | |
| glutathione | required for maximal activity | Ficus benghalensis | |
| glutathione | required for maximal activity | Euphorbia tirucalli | |
| rubber particle | - |
Parthenium argentatum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Ficus elastica | |
| Mg2+ | required for activity | Parthenium argentatum | |
| Mg2+ | required for activity | Parthenium hysterophorus | |
| Mg2+ | required for activity | Ficus lyrata | |
| Mg2+ | required for activity | Ficus benjamina | |
| Mg2+ | required for activity | Ficus deltoidea | |
| Mg2+ | required for activity | Ficus decora | |
| Mg2+ | required for activity | Ficus benghalensis | |
| Mg2+ | required for activity | Euphorbia tirucalli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euphorbia tirucalli | - |
- |
- |
| Ficus benghalensis | - |
- |
- |
| Ficus benjamina | - |
- |
- |
| Ficus decora | - |
- |
- |
| Ficus deltoidea | - |
- |
- |
| Ficus elastica | - |
- |
- |
| Ficus lyrata | - |
- |
- |
| Parthenium argentatum | - |
guayule | - |
| Parthenium hysterophorus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, Sephadex G-25, DEAE-cellulose, partial purification | Parthenium argentatum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| latex | - |
Parthenium argentatum | - |
| leaf | - |
Parthenium argentatum | - |
| mesophyll | - |
Parthenium argentatum | - |
| stem | - |
Parthenium argentatum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000695 | - |
partially purified enzyme | Parthenium argentatum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isopentenyl diphosphate + cis-1,4-polyisoprene | of rubber particles | Ficus elastica | diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
| isopentenyl diphosphate + cis-1,4-polyisoprene | of rubber particles | Parthenium argentatum | diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
| isopentenyl diphosphate + cis-1,4-polyisoprene | of rubber particles | Parthenium hysterophorus | diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
| isopentenyl diphosphate + cis-1,4-polyisoprene | of rubber particles | Ficus lyrata | diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
| isopentenyl diphosphate + cis-1,4-polyisoprene | of rubber particles | Ficus benjamina | diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
| isopentenyl diphosphate + cis-1,4-polyisoprene | of rubber particles | Ficus deltoidea | diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
| isopentenyl diphosphate + cis-1,4-polyisoprene | of rubber particles | Ficus decora | diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
| isopentenyl diphosphate + cis-1,4-polyisoprene | of rubber particles | Ficus benghalensis | diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
| isopentenyl diphosphate + cis-1,4-polyisoprene | of rubber particles | Euphorbia tirucalli | diphosphate + a poly-cis-polyprenyl diphosphate longer by one C5 unit | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Ficus elastica |
| 30 | - |
assay at | Parthenium argentatum |
| 30 | - |
assay at | Parthenium hysterophorus |
| 30 | - |
assay at | Ficus lyrata |
| 30 | - |
assay at | Ficus benjamina |
| 30 | - |
assay at | Ficus deltoidea |
| 30 | - |
assay at | Ficus decora |
| 30 | - |
assay at | Ficus benghalensis |
| 30 | - |
assay at | Euphorbia tirucalli |
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