Literature summary for 2.5.1.2 extracted from

Costello, C.A.; Kelleher, N.L.; Abe, M.; McLafferty, F.W.; Begley, T.P.
Mechanistic studies on thiaminase I. Overexpression and identification of the active site nucleophile (1996), J. Biol. Chem., 271, 3445-3452.

Search for more literature for 2.5.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
cloned in Escherichia coli with Gly6 changed to Ala6 with no apparent effect	Paenibacillus thiaminolyticus

Protein Variants

Protein Variants	Comment	Organism
C113S	inactive mutant at the active nucleophilic site	Paenibacillus thiaminolyticus

Inhibitors

Inhibitors	Comment	Organism	Structure
4-Amino-6-chloro-2-methylpyrimidine	inhibitory	Paenibacillus thiaminolyticus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0037	-	thiamine	-	Paenibacillus thiaminolyticus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
42000	-	SDS-PAGE	Paenibacillus thiaminolyticus

Organism

Organism	UniProt	Comment	Textmining
Paenibacillus thiaminolyticus	P45741	cloned in Escherichia coli	-

Oxidation Stability

Oxidation Stability	Organism
addition of dithiothreitol, 2 mM, reduces the loss of activity	Paenibacillus thiaminolyticus

Purification (Commentary)

Purification (Comment)	Organism
by ammonium precipitation, DEAE column, Cibacron blue dye affinity column	Paenibacillus thiaminolyticus

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	-	Paenibacillus thiaminolyticus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
thiamine + veratrylamine	the reaction can occur without base substrate	Paenibacillus thiaminolyticus	bis-(4-amino-2-methyl)pyrimidinyl-3,4-dimethoxybenzylamine + 4-methyl-5-(2-hydroxyethyl)-thiazole	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 42000, SDS-polyacrylamide gel electrophoresis	Paenibacillus thiaminolyticus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	in 50 mM sodium phosphate at pH 7	Paenibacillus thiaminolyticus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
7	-	50 mM sodium phosphate buffer, 2 mM dithiothreitol, 2 mM EDTA	Paenibacillus thiaminolyticus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
7.7	-	4-Amino-6-chloro-2-methylpyrimidine	-	Paenibacillus thiaminolyticus

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Release 2023.1 (January 2023)