The purification and properties of a thiaminase I enzyme from nardoo (Marsilea Drumondii)
McCleary, B.V.; Chick, B.F.; Phytochemistry 16, 207-213 (1977)
No PubMed abstract available
Data extracted from this reference:
Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
complete reactivation at 3.3 mM but inhibition at 33 mM
Marsilea drummondii
cysteine
complete reactivation at 3.3 mM but inhibition at 33 mM
Marsilea drummondii
Thioglycollate
greater reactivation at 33 mM than at 3.3 mM
Marsilea drummondii
thiosulphate
greater reactivation at 33 mM than at 3.3 mM
Marsilea drummondii
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
100% inhibition at 1 mM
Marsilea drummondii
Cu2+
50% of inhibition at 1 mM
Marsilea drummondii
Fe2+
70% of inhibition at 1 mM
Marsilea drummondii
HgCl2
potent inhibitor at 0.001 mM
Cheilanthes sieberi
HgCl2
potent inhibitor at 0.001 mM
Marsilea drummondii
HgCl2
potent inhibitor at 0.001 mM
Pteridium esculentum
HgCl2
potent inhibitor at 0.001 mM
Velesunio ambiguus
Mersalyl acid
potent inhibitor at 0.001 mM
Cheilanthes sieberi
Mersalyl acid
potent inhibitor at 0.001 mM
Marsilea drummondii
Mersalyl acid
potent inhibitor at 0.001 mM
Pteridium esculentum
Mersalyl acid
potent inhibitor at 0.001 mM
Velesunio ambiguus
Mn2+
no inhibition
Marsilea drummondii
additional information
not inhibited by EDTA
Marsilea drummondii
p-chloromercuribenzoate
complete inhibition at 0.001 mM
Cheilanthes sieberi
p-chloromercuribenzoate
complete inhibition at 0.001 mM
Marsilea drummondii
p-chloromercuribenzoate
complete inhibition at 0.001 mM
Pteridium esculentum
p-chloromercuribenzoate
complete inhibition at 0.001 mM
Velesunio ambiguus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
thiamine
measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate
Marsilea drummondii
0.003
-
thiamine
measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate
Pteridium esculentum
0.003
-
thiamine
measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate
Cheilanthes sieberi
0.003
-
thiamine
measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate
Velesunio ambiguus
0.5
-
beta-picoline
-
Marsilea drummondii
0.7
-
Pyridine
-
Pteridium esculentum
0.7
-
Pyridine
-
Velesunio ambiguus
0.9
-
nicotinic acid
-
Velesunio ambiguus
0.9
-
gamma-Picoline
-
Marsilea drummondii
1.4
-
aniline
lower maximal activity than pyridine and nicotinic acid
Marsilea drummondii
1.4
-
aniline
lower maximal activity than pyridine and nicotinic acid
Pteridium esculentum
1.7
-
m-phenylene diamine
-
Marsilea drummondii
1.7
-
o-phenylene diamine
-
Marsilea drummondii
2
-
aniline
lower maximal activity than pyridine and nicotinic acid
Velesunio ambiguus
2.4
-
m-Toluidine
-
Marsilea drummondii
2.5
-
Pyridine
-
Marsilea drummondii
2.8
-
3-hydroxyaniline
-
Marsilea drummondii
2.9
-
3-aminopyridine
-
Marsilea drummondii
3.3
-
Quinoline
-
Marsilea drummondii
3.3
-
alpha-picoline
-
Marsilea drummondii
3.3
-
p-phenylene diamine
-
Marsilea drummondii
3.4
-
gamma-picolinic acid
-
Marsilea drummondii
4.2
-
2-hydroxyaniline
-
Marsilea drummondii
5
-
Pyridine
-
Cheilanthes sieberi
5
-
o-mercaptobenzoic acid
best cosubstrate
Marsilea drummondii
5.1
-
aniline
lower maximal activity than pyridine and nicotinic acid
Cheilanthes sieberi
6.4
-
o-toluidine
-
Marsilea drummondii
9.5
-
adenine
-
Marsilea drummondii
12.5
-
2-aminopyridine
-
Marsilea drummondii
15.4
-
4-aminopyridine
-
Marsilea drummondii
20
-
4-Hydroxyaniline
-
Marsilea drummondii
21.2
-
alpha-picolinic acid
-
Marsilea drummondii
25
-
nicotinic acid
-
Marsilea drummondii
25
-
beta-picolinic acid
-
Marsilea drummondii
29
-
nicotinic acid
-
Pteridium esculentum
32
-
methylamine
-
Marsilea drummondii
33
-
L-cysteine
-
Marsilea drummondii
37
-
o-aminobenzoic acid
-
Marsilea drummondii
37
-
m-aminobenzoic acid
-
Marsilea drummondii
38
-
6-aminohexanoic acid
-
Marsilea drummondii
50
-
Sulfanilic acid
-
Marsilea drummondii
50
-
3-nitroaniline
-
Marsilea drummondii
55
-
nicotinic acid
-
Cheilanthes sieberi
77
-
sulfanilamide
-
Marsilea drummondii
111
-
proline
-
Marsilea drummondii
125
-
imidazole
-
Marsilea drummondii
200
-
Tris
-
Marsilea drummondii
200
-
p-Aminobenzoic acid
-
Marsilea drummondii
400
-
hydroxyproline
-
Marsilea drummondii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
Ca2+, Mo+, Mn2+, Zn2+, Mg2+ have no effect on activity
Marsilea drummondii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
93000
-
gel filtration
Pteridium esculentum
107000
-
gel filtration
Cheilanthes sieberi
110000
-
gel filtration
Velesunio ambiguus
115000
-
gel filtration
Marsilea drummondii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
thiamine + adenine
Marsilea drummondii
-
?
-
-
?
thiamine + hydroxyproline
Marsilea drummondii
-
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
-
-
?
thiamine + hydroxyproline
Pteridium esculentum
-
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
-
-
?
thiamine + proline
Marsilea drummondii
-
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
-
-
?
thiamine + proline
Pteridium esculentum
-
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Cheilanthes sieberi
-
rock fern
-
Marsilea angustifolia
-
-
-
Marsilea drummondii
-
nardoo
-
Marsilea mutica
-
-
-
Pteridium esculentum
-
bracken fern
-
Velesunio ambiguus
-
freshwater mussel
-
Purification (Commentary)
Purification (Commentary)
Organism
by DEAE-cellulose chromatrography, ultrogel AcA 34 column, Sephadex G-100 column and phosphocellulose column
Marsilea drummondii
Source Tissue
Source Tissue
Commentary
Organism
Textmining
frond
extremely high levels of the enzyme, lush fronds have much more enzyme than old fronds
Marsilea drummondii
-
frond
activity found in lush fronds
Marsilea mutica
-
frond
activity found in lush fronds
Cheilanthes sieberi
-
frond
very low activity in lush fronds
Marsilea angustifolia
-
frond
very low activity in youngs and old fronds
Pteridium esculentum
-
root
lush growth
Marsilea drummondii
-
tuber
very low activity
Pteridium esculentum
-
whole body
activity found
Velesunio ambiguus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
thiamine + 2-aminopyridine
-
636851
Marsilea drummondii
?
-
-
-
?
thiamine + 2-hydroxyaniline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-hydroxyphenyl)amine
-
-
-
?
thiamine + 2-mercaptobenzoic acid
-
636851
Marsilea drummondii
?
-
-
-
?
thiamine + 2-methylbenzeneamine
i.e. o-toluidine
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-methylphenyl)amine
-
-
-
?
thiamine + 2-pyridinecarboxylic acid
i.e. alpha-picolinic acid
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxypyridinium
-
-
-
?
thiamine + 3-aminopyridine
-
636851
Marsilea drummondii
?
-
-
-
?
thiamine + 3-hydroxyaniline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-hydroxyphenyl)amine
-
-
-
?
thiamine + 3-methylbenzeneamine
i.e. m-toluidine
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-methylphenyl)amine
-
-
-
?
thiamine + 3-nitroaniline
-
636851
Marsilea drummondii
?
-
-
-
?
thiamine + 3-pyridinecarboxylic acid
i.e. beta-picolinic acid
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-3-carboxypyridinium
-
-
-
?
thiamine + 4-aminopyridine
-
636851
Marsilea drummondii
?
-
-
-
?
thiamine + 4-hydroxyaniline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-hydroxyphenyl)amine
-
-
-
?
thiamine + 4-methylbenzeneamine
i.e. p-toluidine
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-methylphenyl)amine
-
-
-
?
thiamine + 4-pyridinecarboxylic acid
i.e. gamma-picolinic acid
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-carboxypyridinium
-
-
-
?
thiamine + 6-aminohexanoic acid
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 6-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]hexanoic acid
-
-
-
?
thiamine + adenine
-
636851
Marsilea drummondii
?
-
-
-
?
thiamine + alpha-picoline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]alpha-picolinium
-
-
-
?
thiamine + aniline
-
636851
Marsilea drummondii
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Marsilea drummondii
?
thiamine + aniline
-
636851
Pteridium esculentum
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Pteridium esculentum
?
thiamine + aniline
-
636851
Cheilanthes sieberi
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Cheilanthes sieberi
?
thiamine + aniline
-
636851
Velesunio ambiguus
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Velesunio ambiguus
?
thiamine + beta-picoline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]beta-picolinium
-
-
-
?
thiamine + gamma-picoline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]gamma-picolinium
-
-
-
?
thiamine + hydroxyproline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
-
-
-
?
thiamine + hydroxyproline
-
636851
Pteridium esculentum
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
-
-
-
?
thiamine + imidazole
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 5-(1H-imidazol-1-yl-methyl)-2-methylpyrimidin-4-amine
-
-
-
?
thiamine + L-cysteine
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-ethylpyrimidin-5-yl)methyl]amino]-3-mercaptopropanoic acid
-
-
-
?
thiamine + m-aminobenzoic acid
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 3-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid
-
-
-
?
thiamine + m-phenylene diamine
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-aminophenyl)amine
-
-
-
?
thiamine + methylamine
-
636851
Marsilea drummondii
?
-
-
-
?
thiamine + nicotinic acid
-
636851
Pteridium esculentum
?
-
-
-
?
thiamine + nicotinic acid
-
636851
Cheilanthes sieberi
?
-
-
-
?
thiamine + nicotinic acid
-
636851
Velesunio ambiguus
?
-
-
-
?
thiamine + o-aminobenzoic acid
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid
-
-
-
?
thiamine + o-phenylene diamine
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-aminophenyl)amine
-
-
-
?
thiamine + p-aminobenzoic acid
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 4-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid
-
-
-
?
thiamine + p-phenylene diamine
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-aminophenyl)amine
-
-
-
?
thiamine + proline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
-
-
-
?
thiamine + proline
-
636851
Pteridium esculentum
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
-
-
-
?
thiamine + pyridine
-
636851
Marsilea drummondii
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Marsilea drummondii
?
thiamine + pyridine
-
636851
Pteridium esculentum
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Pteridium esculentum
?
thiamine + pyridine
-
636851
Cheilanthes sieberi
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Cheilanthes sieberi
?
thiamine + pyridine
-
636851
Velesunio ambiguus
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Velesunio ambiguus
?
thiamine + quinoline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]quinolinium
-
-
-
?
thiamine + sulfanilamide
-
636851
Marsilea drummondii
?
-
-
-
?
thiamine + sulfanilic acid
-
636851
Marsilea drummondii
?
-
-
-
?
thiamine + Tris
-
636851
Marsilea drummondii
?
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Marsilea drummondii
8
9
-
Pteridium esculentum
8
9
-
Cheilanthes sieberi
8
9
-
Velesunio ambiguus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
3
12
-
Marsilea drummondii
3
12
-
Pteridium esculentum
3
12
-
Cheilanthes sieberi
3
12
-
Velesunio ambiguus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
3
12
stable in this range
Marsilea drummondii
3
12
stable in this range
Pteridium esculentum
3
12
stable in this range
Cheilanthes sieberi
3
12
stable in this range
Velesunio ambiguus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
complete reactivation at 3.3 mM but inhibition at 33 mM
Marsilea drummondii
cysteine
complete reactivation at 3.3 mM but inhibition at 33 mM
Marsilea drummondii
Thioglycollate
greater reactivation at 33 mM than at 3.3 mM
Marsilea drummondii
thiosulphate
greater reactivation at 33 mM than at 3.3 mM
Marsilea drummondii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
100% inhibition at 1 mM
Marsilea drummondii
Cu2+
50% of inhibition at 1 mM
Marsilea drummondii
Fe2+
70% of inhibition at 1 mM
Marsilea drummondii
HgCl2
potent inhibitor at 0.001 mM
Marsilea drummondii
HgCl2
potent inhibitor at 0.001 mM
Pteridium esculentum
HgCl2
potent inhibitor at 0.001 mM
Cheilanthes sieberi
HgCl2
potent inhibitor at 0.001 mM
Velesunio ambiguus
Mersalyl acid
potent inhibitor at 0.001 mM
Marsilea drummondii
Mersalyl acid
potent inhibitor at 0.001 mM
Pteridium esculentum
Mersalyl acid
potent inhibitor at 0.001 mM
Cheilanthes sieberi
Mersalyl acid
potent inhibitor at 0.001 mM
Velesunio ambiguus
Mn2+
no inhibition
Marsilea drummondii
additional information
not inhibited by EDTA
Marsilea drummondii
p-chloromercuribenzoate
complete inhibition at 0.001 mM
Marsilea drummondii
p-chloromercuribenzoate
complete inhibition at 0.001 mM
Pteridium esculentum
p-chloromercuribenzoate
complete inhibition at 0.001 mM
Cheilanthes sieberi
p-chloromercuribenzoate
complete inhibition at 0.001 mM
Velesunio ambiguus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
thiamine
measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate
Marsilea drummondii
0.003
-
thiamine
measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate
Pteridium esculentum
0.003
-
thiamine
measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate
Cheilanthes sieberi
0.003
-
thiamine
measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate
Velesunio ambiguus
0.5
-
beta-picoline
-
Marsilea drummondii
0.7
-
Pyridine
-
Pteridium esculentum
0.7
-
Pyridine
-
Velesunio ambiguus
0.9
-
nicotinic acid
-
Velesunio ambiguus
0.9
-
gamma-Picoline
-
Marsilea drummondii
1.4
-
aniline
lower maximal activity than pyridine and nicotinic acid
Marsilea drummondii
1.4
-
aniline
lower maximal activity than pyridine and nicotinic acid
Pteridium esculentum
1.7
-
m-phenylene diamine
-
Marsilea drummondii
1.7
-
o-phenylene diamine
-
Marsilea drummondii
2
-
aniline
lower maximal activity than pyridine and nicotinic acid
Velesunio ambiguus
2.4
-
m-Toluidine
-
Marsilea drummondii
2.5
-
Pyridine
-
Marsilea drummondii
2.8
-
3-hydroxyaniline
-
Marsilea drummondii
2.9
-
3-aminopyridine
-
Marsilea drummondii
3.3
-
Quinoline
-
Marsilea drummondii
3.3
-
alpha-picoline
-
Marsilea drummondii
3.3
-
p-phenylene diamine
-
Marsilea drummondii
3.4
-
gamma-picolinic acid
-
Marsilea drummondii
4.2
-
2-hydroxyaniline
-
Marsilea drummondii
5
-
Pyridine
-
Cheilanthes sieberi
5
-
o-mercaptobenzoic acid
best cosubstrate
Marsilea drummondii
5.1
-
aniline
lower maximal activity than pyridine and nicotinic acid
Cheilanthes sieberi
6.4
-
o-toluidine
-
Marsilea drummondii
9.5
-
adenine
-
Marsilea drummondii
12.5
-
2-aminopyridine
-
Marsilea drummondii
15.4
-
4-aminopyridine
-
Marsilea drummondii
20
-
4-Hydroxyaniline
-
Marsilea drummondii
21.2
-
alpha-picolinic acid
-
Marsilea drummondii
25
-
nicotinic acid
-
Marsilea drummondii
25
-
beta-picolinic acid
-
Marsilea drummondii
29
-
nicotinic acid
-
Pteridium esculentum
32
-
methylamine
-
Marsilea drummondii
33
-
L-cysteine
-
Marsilea drummondii
37
-
o-aminobenzoic acid
-
Marsilea drummondii
37
-
m-aminobenzoic acid
-
Marsilea drummondii
38
-
6-aminohexanoic acid
-
Marsilea drummondii
50
-
Sulfanilic acid
-
Marsilea drummondii
50
-
3-nitroaniline
-
Marsilea drummondii
55
-
nicotinic acid
-
Cheilanthes sieberi
77
-
sulfanilamide
-
Marsilea drummondii
111
-
proline
-
Marsilea drummondii
125
-
imidazole
-
Marsilea drummondii
200
-
Tris
-
Marsilea drummondii
200
-
p-Aminobenzoic acid
-
Marsilea drummondii
400
-
hydroxyproline
-
Marsilea drummondii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
Ca2+, Mo+, Mn2+, Zn2+, Mg2+ have no effect on activity
Marsilea drummondii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
93000
-
gel filtration
Pteridium esculentum
107000
-
gel filtration
Cheilanthes sieberi
110000
-
gel filtration
Velesunio ambiguus
115000
-
gel filtration
Marsilea drummondii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
thiamine + adenine
Marsilea drummondii
-
?
-
-
?
thiamine + hydroxyproline
Marsilea drummondii
-
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
-
-
?
thiamine + hydroxyproline
Pteridium esculentum
-
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
-
-
?
thiamine + proline
Marsilea drummondii
-
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
-
-
?
thiamine + proline
Pteridium esculentum
-
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
by DEAE-cellulose chromatrography, ultrogel AcA 34 column, Sephadex G-100 column and phosphocellulose column
Marsilea drummondii
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
frond
extremely high levels of the enzyme, lush fronds have much more enzyme than old fronds
Marsilea drummondii
-
frond
activity found in lush fronds
Marsilea mutica
-
frond
activity found in lush fronds
Cheilanthes sieberi
-
frond
very low activity in lush fronds
Marsilea angustifolia
-
frond
very low activity in youngs and old fronds
Pteridium esculentum
-
root
lush growth
Marsilea drummondii
-
tuber
very low activity
Pteridium esculentum
-
whole body
activity found
Velesunio ambiguus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
thiamine + 2-aminopyridine
-
636851
Marsilea drummondii
?
-
-
-
?
thiamine + 2-hydroxyaniline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-hydroxyphenyl)amine
-
-
-
?
thiamine + 2-mercaptobenzoic acid
-
636851
Marsilea drummondii
?
-
-
-
?
thiamine + 2-methylbenzeneamine
i.e. o-toluidine
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-methylphenyl)amine
-
-
-
?
thiamine + 2-pyridinecarboxylic acid
i.e. alpha-picolinic acid
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxypyridinium
-
-
-
?
thiamine + 3-aminopyridine
-
636851
Marsilea drummondii
?
-
-
-
?
thiamine + 3-hydroxyaniline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-hydroxyphenyl)amine
-
-
-
?
thiamine + 3-methylbenzeneamine
i.e. m-toluidine
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-methylphenyl)amine
-
-
-
?
thiamine + 3-nitroaniline
-
636851
Marsilea drummondii
?
-
-
-
?
thiamine + 3-pyridinecarboxylic acid
i.e. beta-picolinic acid
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-3-carboxypyridinium
-
-
-
?
thiamine + 4-aminopyridine
-
636851
Marsilea drummondii
?
-
-
-
?
thiamine + 4-hydroxyaniline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-hydroxyphenyl)amine
-
-
-
?
thiamine + 4-methylbenzeneamine
i.e. p-toluidine
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-methylphenyl)amine
-
-
-
?
thiamine + 4-pyridinecarboxylic acid
i.e. gamma-picolinic acid
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-carboxypyridinium
-
-
-
?
thiamine + 6-aminohexanoic acid
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 6-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]hexanoic acid
-
-
-
?
thiamine + adenine
-
636851
Marsilea drummondii
?
-
-
-
?
thiamine + alpha-picoline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]alpha-picolinium
-
-
-
?
thiamine + aniline
-
636851
Marsilea drummondii
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Marsilea drummondii
?
thiamine + aniline
-
636851
Pteridium esculentum
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Pteridium esculentum
?
thiamine + aniline
-
636851
Cheilanthes sieberi
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Cheilanthes sieberi
?
thiamine + aniline
-
636851
Velesunio ambiguus
5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Velesunio ambiguus
?
thiamine + beta-picoline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]beta-picolinium
-
-
-
?
thiamine + gamma-picoline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]gamma-picolinium
-
-
-
?
thiamine + hydroxyproline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
-
-
-
?
thiamine + hydroxyproline
-
636851
Pteridium esculentum
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid
-
-
-
?
thiamine + imidazole
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 5-(1H-imidazol-1-yl-methyl)-2-methylpyrimidin-4-amine
-
-
-
?
thiamine + L-cysteine
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-ethylpyrimidin-5-yl)methyl]amino]-3-mercaptopropanoic acid
-
-
-
?
thiamine + m-aminobenzoic acid
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 3-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid
-
-
-
?
thiamine + m-phenylene diamine
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-aminophenyl)amine
-
-
-
?
thiamine + methylamine
-
636851
Marsilea drummondii
?
-
-
-
?
thiamine + nicotinic acid
-
636851
Pteridium esculentum
?
-
-
-
?
thiamine + nicotinic acid
-
636851
Cheilanthes sieberi
?
-
-
-
?
thiamine + nicotinic acid
-
636851
Velesunio ambiguus
?
-
-
-
?
thiamine + o-aminobenzoic acid
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid
-
-
-
?
thiamine + o-phenylene diamine
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-aminophenyl)amine
-
-
-
?
thiamine + p-aminobenzoic acid
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 4-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid
-
-
-
?
thiamine + p-phenylene diamine
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-aminophenyl)amine
-
-
-
?
thiamine + proline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
-
-
-
?
thiamine + proline
-
636851
Pteridium esculentum
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid
-
-
-
?
thiamine + pyridine
-
636851
Marsilea drummondii
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Marsilea drummondii
?
thiamine + pyridine
-
636851
Pteridium esculentum
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Pteridium esculentum
?
thiamine + pyridine
-
636851
Cheilanthes sieberi
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Cheilanthes sieberi
?
thiamine + pyridine
-
636851
Velesunio ambiguus
heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
636851
Velesunio ambiguus
?
thiamine + quinoline
-
636851
Marsilea drummondii
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]quinolinium
-
-
-
?
thiamine + sulfanilamide
-
636851
Marsilea drummondii
?
-
-
-
?
thiamine + sulfanilic acid
-
636851
Marsilea drummondii
?
-
-
-
?
thiamine + Tris
-
636851
Marsilea drummondii
?
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Marsilea drummondii
8
9
-
Pteridium esculentum
8
9
-
Cheilanthes sieberi
8
9
-
Velesunio ambiguus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
3
12
-
Marsilea drummondii
3
12
-
Pteridium esculentum
3
12
-
Cheilanthes sieberi
3
12
-
Velesunio ambiguus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
3
12
stable in this range
Marsilea drummondii
3
12
stable in this range
Pteridium esculentum
3
12
stable in this range
Cheilanthes sieberi
3
12
stable in this range
Velesunio ambiguus
Other publictions for EC 2.5.1.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
759218
Boggs
-
Phylogeny and foraging mode c ...
Notropis hudsonius, Catostomus commersonii, Alosa pseudoharengus, Fundulus diaphanus, Campostoma anomalum, Luxilus cornutus, Exoglossum maxillingua, Semotilus corporalis, Rhinichthys cataractae, Etheostoma olmstedi, Dorosoma cepedianum
Freshw. Sci.
38
605-615
2019
-
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-
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-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11
11
-
-
-
758667
Sannino
Thiaminase I provides a growt ...
Burkholderia thailandensis, Burkholderia thailandensis ATCC 700388
Appl. Environ. Microbiol.
84
e01268-18
2018
-
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-
-
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
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737986
Lau
Metabolomic profiling of Burkh ...
Burkholderia pseudomallei, Burkholderia thailandensis
Cell Biosci.
5
26
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
738782
Blakeslee
-
Thiaminase activity in native ...
Strophitus undulatus, Elliptio complanata
J. Great Lakes Res.
41
516-519
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739509
Kraft
A rapid method for assaying th ...
Paenibacillus thiaminolyticus, Paenibacillus thiaminolyticus 8103
PLoS ONE
9
e92688
2014
-
1
-
-
-
-
-
-
-
-
-
-
-
10
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
737686
Sikowitz
Structure of a Clostridium bot ...
Clostridium botulinum
Biochemistry
52
7830-7839
2013
-
-
-
1
6
-
-
6
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
1
6
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
6
-
-
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-
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6
6
723037
Liu
Pharmacologic properties of po ...
Paenibacillus thiaminolyticus
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341
775-783
2012
-
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-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
711655
Liu
Sensitivity of breast cancer c ...
Paenibacillus thiaminolyticus
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66
171-179
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-
1
1
-
-
-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
704238
Wistbacka
Thiaminase activity of crucian ...
Carassius carassius
J. Aquat. Anim. Health
21
217-228
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
18
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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2
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1
1
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-
-
685198
Soriano
Structural similarities betwee ...
Paenibacillus thiaminolyticus
Biochemistry
47
1346-1357
2008
-
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1
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1
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688111
Wistbacka
-
Thiaminase activity of Baltic ...
Clupea harengus, Sprattus sprattus, Gasterosteus aculeatus
J. Fish Biol.
72
787-802
2008
-
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3
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1
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-
699702
Nishimune
Studies on the polymorphism of ...
Fistularia petimba
J. Nutr. Sci. Vitaminol.
54
339-346
2008
-
-
-
-
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1
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1
1
1
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11
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1
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1
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9
1
1
2
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3
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1
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1
1
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1
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1
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9
1
2
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3
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1
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684401
Hanes
An assay for thiaminase I in c ...
Paenibacillus thiaminolyticus
Anal. Biochem.
368
33-38
2007
-
-
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4
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2
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1
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2
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2
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4
1
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4
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2
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2
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4
1
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2
2
636861
Wistbacka
-
Thiaminase activity of gastroi ...
Clupea harengus, Salmo salar
J. Fish Biol.
60
1031-1042
2002
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2
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2
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4
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636859
Wu
-
Mechanistic studies on thiamin ...
Paenibacillus thiaminolyticus
Bioorg. Chem.
28
45-48
2000
-
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1
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1
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1
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636860
Bos
Some molecular and enzymatic p ...
Cyprinus carpio
J. Protein Chem.
19
75-84
2000
-
-
-
-
-
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1
-
1
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6
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1
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5
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20
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1
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1
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1
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1
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5
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20
-
1
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-
1
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-
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-
636858
Campobasso
Crystallization and preliminar ...
Paenibacillus thiaminolyticus
Acta Crystallogr. Sect. D
54
448-450
1998
-
-
-
1
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7
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1
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1
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1
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1
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-
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-
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-
636854
Kelleher
Identification of modification ...
Paenibacillus thiaminolyticus
J. Biol. Chem.
272
32215-32220
1997
-
-
-
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1
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1
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-
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1
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-
636853
Costello
Mechanistic studies on thiamin ...
Paenibacillus thiaminolyticus
J. Biol. Chem.
271
3445-3452
1996
-
-
1
-
1
-
1
1
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1
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7
1
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1
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1
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1
1
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1
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1
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1
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1
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1
1
1
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1
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1
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1
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1
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-
1
1
1
-
-
-
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-
1
-
-
-
-
-
-
-
636857
Ruml
-
Purification of thiaminase I f ...
Paenibacillus thiaminolyticus, Paenibacillus thiaminolyticus DBM 1068
Potravinarske Vedy
13
181-187
1995
-
1
-
-
-
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-
1
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2
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1
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1
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1
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1
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1
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-
-
636856
Sato
-
Subcellular localization of th ...
Cyprinus carpio
Comp. Biochem. Physiol. A
108A
31-38
1994
-
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-
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1
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1
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2
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-
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-
636855
Fabre
-
Thiaminase activity in Equiset ...
Equisetum arvense
Plant. Med. Phytother.
26
190-197
1993
-
1
-
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1
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1
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2
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1
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1
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1
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1
2
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1
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-
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-
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-
636852
Hutter
Inhibition of thiaminase I fro ...
Paenibacillus thiaminolyticus, Paenibacillus thiaminolyticus BMM
Biochemistry
26
1969-1973
1987
-
-
-
-
-
-
3
-
1
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8
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1
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4
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1
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3
1
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1
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1
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-
4
-
-
-
-
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-
-
-
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-
-
-
-
636847
Boyd
-
Studies on thiaminase I activi ...
Megasphaera elsdenii
J. Agric. Sci.
104
637-642
1985
-
-
-
-
-
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1
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1
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1
-
-
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-
-
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-
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-
-
-
-
-
-
636851
McCleary
-
The purification and propertie ...
Cheilanthes sieberi, Marsilea angustifolia, Marsilea drummondii, Marsilea mutica, Pteridium esculentum, Velesunio ambiguus
Phytochemistry
16
207-213
1977
4
-
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-
-
17
49
-
1
4
5
-
6
-
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1
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8
-
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47
-
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4
4
4
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4
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17
-
49
-
1
4
5
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1
-
8
-
-
47
-
-
-
-
-
4
4
4
-
-
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-
-
-
-
636849
Suzuki
Reversible inactivation of ext ...
Paenibacillus thiaminolyticus, Paenibacillus thiaminolyticus YUSM I00I
Biochim. Biophys. Acta
293
111-117
1973
7
-
-
-
-
-
7
-
1
-
-
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-
4
-
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1
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6
-
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2
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1
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7
-
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8
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1
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1
-
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6
-
-
2
-
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-
1
-
-
-
-
-
-
-
636850
Agee
Reversible inactivation of thi ...
Paenibacillus thiaminolyticus
J. Bacteriol.
115
957-965
1973
2
-
-
-
-
-
2
-
1
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3
-
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1
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1
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1
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1
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1
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2
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2
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1
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-
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1
-
1
-
-
1
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
636848
Suzuki
Reversible inactivation of cel ...
Paenibacillus thiaminolyticus
J. Biochem.
72
1053-1055
1972
-
-
-
-
-
-
3
-
1
-
-
-
-
3
-
-
-
-
-
1
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1
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-
1
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-
-
-
-
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-
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-
-
3
-
-
1
-
-
-
-
-
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-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
636846
Wittliff
The extracellular thiaminase I ...
Paenibacillus thiaminolyticus, Paenibacillus thiaminolyticus M
Biochemistry
7
736-744
1968
-
-
-
-
-
-
2
2
1
-
1
-
-
4
-
-
1
-
-
1
-
-
2
-
-
1
2
-
-
-
1
1
-
-
-
-
-
-
-
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-
-
-
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2
-
2
1
-
1
-
-
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-
1
-
1
-
-
2
-
1
2
-
-
-
1
1
-
-
-
-
-
-
-
636845
Fujita
Thiaminase ...
Acanthogobius flavimanus, Anadara inflata, Anguilla japonica, Athyrium nipponicum, Auxis hira, Aneurinibacillus aneurinilyticus, Paenibacillus thiaminolyticus, Turbo cornutus, Struthiopteris nipponica, Carassius auratus, Cyprinus carpio, Celosia crista, Charybdis 6-dentata, Circe scripta, Clava kochi, Corbicula leana, Cristaria plicata, Dicranopteris linearis, Dicranopteris sp., Dryopteris erythrosora, Dryopteris lacera, Eleotris oxycephala, Equisetum arvense, Eviota abax, Gnathopogon mayedae, Haliotis gigantea, Haliotis japonica, Hypomesus olidus, Ischikauia steenackeri, Leucopsarion petersii, Lycopodium clavatum, Mactra sulcataria, Mactra quadrangularis, Meretrix meretrix, Misgurnus anguillicaudatus, Mugil cephalus, no activity in Astroconger myriaster, no activity in Cambaroides japonicus, no activity in Camposcia retusa, no activity in Metapenaeopsis acclivis, no activity in Plecoglossus altivelis, no activity in Squilla oratoria, Odontobutis obscura, Osmunda japonica, Ostrea laperousei, Panulirus japonicus, Paphia philippinarum, Silurus asotus, Leptochilus ellipticus, Polystichum aculeatum, Polystichum falcatum, Polystichum fortunei, Polystichum tsus-simense, Portunus trituberculatus, Pteridium aquilinum, Rhinogobius similis, Solen gouldi, Thiara libertina, Umbonium costatum, Viviparus malleatus, Aneurinibacillus aneurinilyticus BKA, Paenibacillus thiaminolyticus BMM
Adv. Enzymol. Relat. Subj. Biochem.
15
389-421
1954
37
4
-
-
-
-
25
-
-
-
-
-
-
64
-
-
-
-
-
99
-
-
5
-
-
-
9
-
-
9
-
-
-
-
-
-
37
4
-
-
-
-
-
-
25
-
-
-
-
-
-
-
-
-
-
-
99
-
-
5
-
-
9
-
-
9
-
-
-
-
-
-
-
-
-