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Literature summary for 2.5.1.2 extracted from
- The purification and properties of a thiaminase I enzyme from nardoo (Marsilea Drumondii) (1977), Phytochemistry, 16, 207-213.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | complete reactivation at 3.3 mM but inhibition at 33 mM | Marsilea drummondii |  |
| cysteine | complete reactivation at 3.3 mM but inhibition at 33 mM | Marsilea drummondii | |
| Thioglycollate | greater reactivation at 33 mM than at 3.3 mM | Marsilea drummondii | |
| thiosulphate | greater reactivation at 33 mM than at 3.3 mM | Marsilea drummondii | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 100% inhibition at 1 mM | Marsilea drummondii | |
| Cu2+ | 50% of inhibition at 1 mM | Marsilea drummondii | |
| Fe2+ | 70% of inhibition at 1 mM | Marsilea drummondii | |
| HgCl2 | potent inhibitor at 0.001 mM | Cheilanthes sieberi | |
| HgCl2 | potent inhibitor at 0.001 mM | Marsilea drummondii | |
| HgCl2 | potent inhibitor at 0.001 mM | Pteridium esculentum | |
| HgCl2 | potent inhibitor at 0.001 mM | Velesunio ambiguus | |
| Mersalyl acid | potent inhibitor at 0.001 mM | Cheilanthes sieberi | |
| Mersalyl acid | potent inhibitor at 0.001 mM | Marsilea drummondii | |
| Mersalyl acid | potent inhibitor at 0.001 mM | Pteridium esculentum | |
| Mersalyl acid | potent inhibitor at 0.001 mM | Velesunio ambiguus | |
| Mn2+ | no inhibition | Marsilea drummondii | |
| additional information | not inhibited by EDTA | Marsilea drummondii | |
| p-chloromercuribenzoate | complete inhibition at 0.001 mM | Cheilanthes sieberi | |
| p-chloromercuribenzoate | complete inhibition at 0.001 mM | Marsilea drummondii | |
| p-chloromercuribenzoate | complete inhibition at 0.001 mM | Pteridium esculentum | |
| p-chloromercuribenzoate | complete inhibition at 0.001 mM | Velesunio ambiguus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
thiamine | measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate | Marsilea drummondii | |
| 0.003 | - |
thiamine | measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate | Pteridium esculentum | |
| 0.003 | - |
thiamine | measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate | Cheilanthes sieberi | |
| 0.003 | - |
thiamine | measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate | Velesunio ambiguus | |
| 0.5 | - |
beta-picoline | - |
Marsilea drummondii | |
| 0.7 | - |
Pyridine | - |
Pteridium esculentum | |
| 0.7 | - |
Pyridine | - |
Velesunio ambiguus | |
| 0.9 | - |
nicotinic acid | - |
Velesunio ambiguus | |
| 0.9 | - |
gamma-Picoline | - |
Marsilea drummondii | |
| 1.4 | - |
aniline | lower maximal activity than pyridine and nicotinic acid | Marsilea drummondii | |
| 1.4 | - |
aniline | lower maximal activity than pyridine and nicotinic acid | Pteridium esculentum | |
| 1.7 | - |
m-phenylene diamine | - |
Marsilea drummondii | |
| 1.7 | - |
o-phenylene diamine | - |
Marsilea drummondii | |
| 2 | - |
aniline | lower maximal activity than pyridine and nicotinic acid | Velesunio ambiguus | |
| 2.4 | - |
m-Toluidine | - |
Marsilea drummondii | |
| 2.5 | - |
Pyridine | - |
Marsilea drummondii | |
| 2.8 | - |
3-hydroxyaniline | - |
Marsilea drummondii | |
| 2.9 | - |
3-aminopyridine | - |
Marsilea drummondii | |
| 3.3 | - |
Quinoline | - |
Marsilea drummondii | |
| 3.3 | - |
alpha-picoline | - |
Marsilea drummondii | |
| 3.3 | - |
p-phenylene diamine | - |
Marsilea drummondii | |
| 3.4 | - |
gamma-picolinic acid | - |
Marsilea drummondii | |
| 4.2 | - |
2-hydroxyaniline | - |
Marsilea drummondii | |
| 5 | - |
Pyridine | - |
Cheilanthes sieberi | |
| 5 | - |
o-mercaptobenzoic acid | best cosubstrate | Marsilea drummondii | |
| 5.1 | - |
aniline | lower maximal activity than pyridine and nicotinic acid | Cheilanthes sieberi | |
| 6.4 | - |
o-toluidine | - |
Marsilea drummondii | |
| 9.5 | - |
adenine | - |
Marsilea drummondii | |
| 12.5 | - |
2-aminopyridine | - |
Marsilea drummondii | |
| 15.4 | - |
4-aminopyridine | - |
Marsilea drummondii | |
| 20 | - |
4-Hydroxyaniline | - |
Marsilea drummondii | |
| 21.2 | - |
alpha-picolinic acid | - |
Marsilea drummondii | |
| 25 | - |
nicotinic acid | - |
Marsilea drummondii | |
| 25 | - |
beta-picolinic acid | - |
Marsilea drummondii | |
| 29 | - |
nicotinic acid | - |
Pteridium esculentum | |
| 32 | - |
methylamine | - |
Marsilea drummondii | |
| 33 | - |
L-cysteine | - |
Marsilea drummondii | |
| 37 | - |
o-aminobenzoic acid | - |
Marsilea drummondii | |
| 37 | - |
m-aminobenzoic acid | - |
Marsilea drummondii | |
| 38 | - |
6-aminohexanoic acid | - |
Marsilea drummondii | |
| 50 | - |
Sulfanilic acid | - |
Marsilea drummondii | |
| 50 | - |
3-nitroaniline | - |
Marsilea drummondii | |
| 55 | - |
nicotinic acid | - |
Cheilanthes sieberi | |
| 77 | - |
sulfanilamide | - |
Marsilea drummondii | |
| 111 | - |
proline | - |
Marsilea drummondii | |
| 125 | - |
imidazole | - |
Marsilea drummondii | |
| 200 | - |
Tris | - |
Marsilea drummondii | |
| 200 | - |
p-Aminobenzoic acid | - |
Marsilea drummondii | |
| 400 | - |
hydroxyproline | - |
Marsilea drummondii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Ca2+, Mo+, Mn2+, Zn2+, Mg2+ have no effect on activity | Marsilea drummondii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 93000 | - |
gel filtration | Pteridium esculentum |
| 107000 | - |
gel filtration | Cheilanthes sieberi |
| 110000 | - |
gel filtration | Velesunio ambiguus |
| 115000 | - |
gel filtration | Marsilea drummondii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiamine + adenine | Marsilea drummondii | - |
? | - |
? | |
| thiamine + hydroxyproline | Marsilea drummondii | - |
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid | - |
? | |
| thiamine + hydroxyproline | Pteridium esculentum | - |
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid | - |
? | |
| thiamine + proline | Marsilea drummondii | - |
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid | - |
? | |
| thiamine + proline | Pteridium esculentum | - |
4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cheilanthes sieberi | - |
rock fern | - |
| Marsilea angustifolia | - |
- |
- |
| Marsilea drummondii | - |
nardoo | - |
| Marsilea mutica | - |
- |
- |
| Pteridium esculentum | - |
bracken fern | - |
| Velesunio ambiguus | - |
freshwater mussel | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by DEAE-cellulose chromatrography, ultrogel AcA 34 column, Sephadex G-100 column and phosphocellulose column | Marsilea drummondii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| frond | extremely high levels of the enzyme, lush fronds have much more enzyme than old fronds | Marsilea drummondii | - |
| frond | activity found in lush fronds | Marsilea mutica | - |
| frond | activity found in lush fronds | Cheilanthes sieberi | - |
| frond | very low activity in lush fronds | Marsilea angustifolia | - |
| frond | very low activity in youngs and old fronds | Pteridium esculentum | - |
| root | lush growth | Marsilea drummondii | - |
| tuber | very low activity | Pteridium esculentum | - |
| whole body | activity found | Velesunio ambiguus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiamine + 2-aminopyridine | - |
Marsilea drummondii | ? | - |
? | |
| thiamine + 2-hydroxyaniline | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-hydroxyphenyl)amine | - |
? | |
| thiamine + 2-mercaptobenzoic acid | - |
Marsilea drummondii | ? | - |
? | |
| thiamine + 2-methylbenzeneamine | i.e. o-toluidine | Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-methylphenyl)amine | - |
? | |
| thiamine + 2-pyridinecarboxylic acid | i.e. alpha-picolinic acid | Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-carboxypyridinium | - |
? | |
| thiamine + 3-aminopyridine | - |
Marsilea drummondii | ? | - |
? | |
| thiamine + 3-hydroxyaniline | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-hydroxyphenyl)amine | - |
? | |
| thiamine + 3-methylbenzeneamine | i.e. m-toluidine | Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-methylphenyl)amine | - |
? | |
| thiamine + 3-nitroaniline | - |
Marsilea drummondii | ? | - |
? | |
| thiamine + 3-pyridinecarboxylic acid | i.e. beta-picolinic acid | Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-3-carboxypyridinium | - |
? | |
| thiamine + 4-aminopyridine | - |
Marsilea drummondii | ? | - |
? | |
| thiamine + 4-hydroxyaniline | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-hydroxyphenyl)amine | - |
? | |
| thiamine + 4-methylbenzeneamine | i.e. p-toluidine | Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-methylphenyl)amine | - |
? | |
| thiamine + 4-pyridinecarboxylic acid | i.e. gamma-picolinic acid | Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-carboxypyridinium | - |
? | |
| thiamine + 6-aminohexanoic acid | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 6-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]hexanoic acid | - |
? | |
| thiamine + adenine | - |
Marsilea drummondii | ? | - |
? | |
| thiamine + alpha-picoline | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]alpha-picolinium | - |
? | |
| thiamine + aniline | - |
Marsilea drummondii | 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
| thiamine + aniline | - |
Pteridium esculentum | 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
| thiamine + aniline | - |
Cheilanthes sieberi | 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
| thiamine + aniline | - |
Velesunio ambiguus | 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
| thiamine + beta-picoline | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]beta-picolinium | - |
? | |
| thiamine + gamma-picoline | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]gamma-picolinium | - |
? | |
| thiamine + hydroxyproline | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid | - |
? | |
| thiamine + hydroxyproline | - |
Pteridium esculentum | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]4-hydroxypyrrolidine-2-carboxylic acid | - |
? | |
| thiamine + imidazole | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 5-(1H-imidazol-1-yl-methyl)-2-methylpyrimidin-4-amine | - |
? | |
| thiamine + L-cysteine | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-ethylpyrimidin-5-yl)methyl]amino]-3-mercaptopropanoic acid | - |
? | |
| thiamine + m-aminobenzoic acid | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 3-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid | - |
? | |
| thiamine + m-phenylene diamine | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(3-aminophenyl)amine | - |
? | |
| thiamine + methylamine | - |
Marsilea drummondii | ? | - |
? | |
| thiamine + nicotinic acid | - |
Pteridium esculentum | ? | - |
? | |
| thiamine + nicotinic acid | - |
Cheilanthes sieberi | ? | - |
? | |
| thiamine + nicotinic acid | - |
Velesunio ambiguus | ? | - |
? | |
| thiamine + o-aminobenzoic acid | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 2-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid | - |
? | |
| thiamine + o-phenylene diamine | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(2-aminophenyl)amine | - |
? | |
| thiamine + p-aminobenzoic acid | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 4-[[(4-amino-2-methylpyrimidin-5-yl)methyl]amino]benzoic acid | - |
? | |
| thiamine + p-phenylene diamine | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + [(4-amino-2-methylpyrimidin-5-yl)methyl]-N-(4-aminophenyl)amine | - |
? | |
| thiamine + proline | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid | - |
? | |
| thiamine + proline | - |
Pteridium esculentum | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]pyrrolidine-2-carboxylic acid | - |
? | |
| thiamine + pyridine | - |
Marsilea drummondii | heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
| thiamine + pyridine | - |
Pteridium esculentum | heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
| thiamine + pyridine | - |
Cheilanthes sieberi | heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
| thiamine + pyridine | - |
Velesunio ambiguus | heteropyrithiamine + 4-methyl-5-(2-hydroxyethyl)-thiazole | - |
? | |
| thiamine + quinoline | - |
Marsilea drummondii | 4-methyl-5-(2-hydroxyethyl)-thiazole + 1-[(4-amino-2-methylpyrimidin-5-yl)methyl]quinolinium | - |
? | |
| thiamine + sulfanilamide | - |
Marsilea drummondii | ? | - |
? | |
| thiamine + sulfanilic acid | - |
Marsilea drummondii | ? | - |
? | |
| thiamine + Tris | - |
Marsilea drummondii | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Marsilea drummondii |
| 8 | 9 | - |
Pteridium esculentum |
| 8 | 9 | - |
Cheilanthes sieberi |
| 8 | 9 | - |
Velesunio ambiguus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 12 | - |
Marsilea drummondii |
| 3 | 12 | - |
Pteridium esculentum |
| 3 | 12 | - |
Cheilanthes sieberi |
| 3 | 12 | - |
Velesunio ambiguus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 12 | stable in this range | Marsilea drummondii |
| 3 | 12 | stable in this range | Pteridium esculentum |
| 3 | 12 | stable in this range | Cheilanthes sieberi |
| 3 | 12 | stable in this range | Velesunio ambiguus |
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