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Literature summary for 2.5.1.2 extracted from

  • Agee, C.C.; Airth, R.L.
    Reversible inactivation of thiaminase I of Bacillus thiaminolyticus by its primary substrate, thiamine (1973), J. Bacteriol., 115, 957-965.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.2

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol reactivates enzyme less effectively than dithiothreitol Paenibacillus thiaminolyticus
dithiothreitol reactivates 60-100% of the original activity Paenibacillus thiaminolyticus

Inhibitors

Inhibitors Comment Organism Structure
additional information acidic conditions prevent inactivation but low pH does not restore activity after inactivation, quaternary nitrogen linked to a substituted pyrimidine ring through a methylene bridge is shared by compounds that inactivates the enzyme, inactivation alters either the net charge or the structure of the protein or both Paenibacillus thiaminolyticus
thiamine inactivation is proportional to thiamine concentration and the thiamine/enzyme ratio is 1 mol/1 mol Paenibacillus thiaminolyticus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Paenibacillus thiaminolyticus
-
-

Organism

Organism UniProt Comment Textmining
Paenibacillus thiaminolyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Tris-glycine polyacrylamide electrophoresis Paenibacillus thiaminolyticus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Paenibacillus thiaminolyticus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thiamine + aniline one molecule of thiamine is cleaved releasing one molecule of methylhydroxyethylthiazole for one molecule of enzyme Paenibacillus thiaminolyticus 5-(anilinomethyl)-2-methylpyrimidin-4-amine + 4-methyl-5-(2-hydroxyethyl)-thiazole
-
 ?

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25
-
 assay at Paenibacillus thiaminolyticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.8
-
 0.1 M sodium phosphate buffer Paenibacillus thiaminolyticus