Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 (DE3) and Arabidopsis thaliana | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
food industry | the enzyme can be used for cultivation of glyphosate-tolerant plants | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glyphosate | - |
Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
phosphoenolpyruvate | at pH 8.0 and 37°C | Thermotoga maritima | |
0.37 | - |
phosphoenolpyruvate | at pH 8.0 and 22°C | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | Thermotoga maritima | - |
phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA agarose affinity column chromatography | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | - |
Thermotoga maritima | phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44300, SDS-PAGE | Thermotoga maritima |
? | x * 46500, calculated from amino acid sequence | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
5-enolpyruvylshikimate-3-phosphate synthase | - |
Thermotoga maritima |
AroA | - |
Thermotoga maritima |
EPSPS | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 90 | the half-lives of the enzyme at 30, 50, 70 and 90°C are 630, 138, 24 and 9 min, respectively | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Thermotoga maritima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | the enzyme activity is maintained at a pH range of 5.0-9.0, but lost abruptly at pH below 5.0 and above 9.0 | Thermotoga maritima |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | the enzyme is stable from pH 4.0 to 8.0 with more than 60% activity remaining after 1 h incubation | Thermotoga maritima |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
glyphosate | at pH 8.0 and 37°C | Thermotoga maritima | |
3.2 | - |
glyphosate | at pH 8.0 and 22°C | Thermotoga maritima |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
8.4 | - |
at pH 8.0 and 22°C | Thermotoga maritima | glyphosate | |
10 | - |
at pH 8.0 and 37°C | Thermotoga maritima | glyphosate |