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Literature summary for 2.5.1.19 extracted from

  • Wang, L.; Peng, R.; Tian, Y.; Gao, J.; Wang, B.; Yao, Q.
    A thermostable 5-enolpyruvylshikimate-3-phosphate synthase from Thermotoga maritima enhances glyphosate tolerance in Escherichia coli and transgenic Arabidopsis (2019), Extremophiles, 23, 659-667 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 (DE3) and Arabidopsis thaliana Thermotoga maritima

Protein Variants

Protein Variants Comment Organism
food industry the enzyme can be used for cultivation of glyphosate-tolerant plants Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
glyphosate
-
Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
phosphoenolpyruvate at pH 8.0 and 37°C Thermotoga maritima
0.37
-
phosphoenolpyruvate at pH 8.0 and 22°C Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate Thermotoga maritima
-
phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA agarose affinity column chromatography Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate
-
Thermotoga maritima phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
?

Subunits

Subunits Comment Organism
? x * 44300, SDS-PAGE Thermotoga maritima
? x * 46500, calculated from amino acid sequence Thermotoga maritima

Synonyms

Synonyms Comment Organism
5-enolpyruvylshikimate-3-phosphate synthase
-
Thermotoga maritima
AroA
-
Thermotoga maritima
EPSPS
-
Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 90 the half-lives of the enzyme at 30, 50, 70 and 90°C are 630, 138, 24 and 9 min, respectively Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
5 9 the enzyme activity is maintained at a pH range of 5.0-9.0, but lost abruptly at pH below 5.0 and above 9.0 Thermotoga maritima

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 the enzyme is stable from pH 4.0 to 8.0 with more than 60% activity remaining after 1 h incubation Thermotoga maritima

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7
-
glyphosate at pH 8.0 and 37°C Thermotoga maritima
3.2
-
glyphosate at pH 8.0 and 22°C Thermotoga maritima

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
8.4
-
at pH 8.0 and 22°C Thermotoga maritima glyphosate
10
-
at pH 8.0 and 37°C Thermotoga maritima glyphosate