Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 and Isatis indigotica hairy roots | Strobilanthes cusia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glyphosate | - |
Strobilanthes cusia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02167 | - |
phosphoenolpyruvate | at pH 7.0 and 30°C | Strobilanthes cusia |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Strobilanthes cusia | 5829 | - |
plastid | - |
Strobilanthes cusia | 9536 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | Strobilanthes cusia | - |
phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Strobilanthes cusia | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Strobilanthes cusia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Strobilanthes cusia | - |
root | - |
Strobilanthes cusia | - |
stem | highest expression | Strobilanthes cusia | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | - |
Strobilanthes cusia | phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, His-tagged enzyme, SDS-PAGE | Strobilanthes cusia |
? | x * 55330, calculated from amino acid sequence | Strobilanthes cusia |
Synonyms | Comment | Organism |
---|---|---|
5-enolpyruvylshikimate-3-phosphate synthase | - |
Strobilanthes cusia |
EPSPS | - |
Strobilanthes cusia |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000046 | - |
glyphosate | at pH 7.0 and 30°C | Strobilanthes cusia |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Strobilanthes cusia | calculated from amino acid sequence | - |
7.6 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00637 | - |
at pH 7.0 and 30°C | Strobilanthes cusia | glyphosate |
Organism | Comment | Expression |
---|---|---|
Strobilanthes cusia | the enzyme expression is upregulated by methyl jasmonate, salicylic acid and abscisic acid treatment | up |