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Literature summary for 2.5.1.19 extracted from

  • Yu, J.; Zhang, Y.; Ning, S.; Ye, Q.; Tan, H.; Chen, R.; Bu, Q.; Zhang, R.; Gong, P.; Ma, X.; Zhang, L.; Wei, D.
    Molecular cloning and metabolomic characterization of the 5-enolpyruvylshikimate-3-phosphate synthase gene from Baphicacanthus cusia (2019), BMC Plant Biol., 19, 485 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 and Isatis indigotica hairy roots Strobilanthes cusia

Inhibitors

Inhibitors Comment Organism Structure
glyphosate
-
Strobilanthes cusia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02167
-
phosphoenolpyruvate at pH 7.0 and 30°C Strobilanthes cusia

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Strobilanthes cusia 5829
-
plastid
-
Strobilanthes cusia 9536
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate Strobilanthes cusia
-
phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
?

Organism

Organism UniProt Comment Textmining
Strobilanthes cusia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Strobilanthes cusia

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Strobilanthes cusia
-
root
-
Strobilanthes cusia
-
stem highest expression Strobilanthes cusia
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate
-
Strobilanthes cusia phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
?

Subunits

Subunits Comment Organism
? x * 70000, His-tagged enzyme, SDS-PAGE Strobilanthes cusia
? x * 55330, calculated from amino acid sequence Strobilanthes cusia

Synonyms

Synonyms Comment Organism
5-enolpyruvylshikimate-3-phosphate synthase
-
Strobilanthes cusia
EPSPS
-
Strobilanthes cusia

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000046
-
glyphosate at pH 7.0 and 30°C Strobilanthes cusia

pI Value

Organism Comment pI Value Maximum pI Value
Strobilanthes cusia calculated from amino acid sequence
-
7.6

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00637
-
at pH 7.0 and 30°C Strobilanthes cusia glyphosate

Expression

Organism Comment Expression
Strobilanthes cusia the enzyme expression is upregulated by methyl jasmonate, salicylic acid and abscisic acid treatment up