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Literature summary for 2.5.1.19 extracted from
- Expression of the 5-enoylpyruvylshikimate-3-phosphate synthase domain from the Acremonium sp. aroM complex enhances resistance to glyphosate (2018), Biotechnol. Lett., 40, 855-864 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Acremonium sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glyphosate | - |
Acremonium sp. |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.077 | - |
phosphoenolpyruvate | at pH 7.0 and 30°C | Acremonium sp. | |
| 0.144 | - |
3-phosphoshikimate | at pH 7.0 and 30°C | Acremonium sp. | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + 3-phosphoshikimate | Acremonium sp. | - |
phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acremonium sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Acremonium sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + 3-phosphoshikimate | - |
Acremonium sp. | phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Acremonium sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5-enoylpyruvylshikimate-3-phosphate synthase | - |
Acremonium sp. |
| AroA | - |
Acremonium sp. |
| EPSP synthase | - |
Acremonium sp. |
| EPSPS | - |
Acremonium sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Acremonium sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Acremonium sp. |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0082 | - |
glyphosate | at pH 7.0 and 30°C | Acremonium sp. | |
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Release 2023.1 (January 2023)
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