Literature summary for 2.5.1.19 extracted from

Light, S.H.; Krishna, S.N.; Minasov, G.; Anderson, W.F.
An unusual cation-binding site and distinct domain-domain interactions distinguish class II enolpyruvylshikimate-3-phosphate synthases (2016), Biochemistry, 55, 1239-1245 .

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Vibrio cholerae serotype O1
expressed in Escherichia coli BL21(DE3) cells	Coxiella burnetii

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 0.2 M MgCl, 0.1 M HEPES (pH 7.5), and 25% (w/v) PEG 3350	Vibrio cholerae serotype O1
sitting drop vapor diffusion method, using either 0.1 M trisodium citrate (pH 4.0) and 0.8 M ammonium sulfate (unliganded-open enzyme form) or 4.3 M NaCl and 0.1 M HEPES (pH 7.5) (unliganded-closed enzyme form) or 0.1 M Bis-Tris (pH 6.5) and 25% (w/v) PEG3350 (in complex with 3-phosphoshikimate and glyphosate) or 0.2 M ammonium sulfate, 0.1 M HEPES (pH 7.5), and 25% (w/v) PEG 3350 (in complex with K+, phosphate and 3-phosphoshikimate)	Coxiella burnetii

Crystallization (Comment)

Organism

sitting drop vapor diffusion method, using 0.2 M MgCl, 0.1 M HEPES (pH 7.5), and 25% (w/v) PEG 3350

Vibrio cholerae serotype O1

sitting drop vapor diffusion method, using either 0.1 M trisodium citrate (pH 4.0) and 0.8 M ammonium sulfate (unliganded-open enzyme form) or 4.3 M NaCl and 0.1 M HEPES (pH 7.5) (unliganded-closed enzyme form) or 0.1 M Bis-Tris (pH 6.5) and 25% (w/v) PEG3350 (in complex with 3-phosphoshikimate and glyphosate) or 0.2 M ammonium sulfate, 0.1 M HEPES (pH 7.5), and 25% (w/v) PEG 3350 (in complex with K+, phosphate and 3-phosphoshikimate)

Coxiella burnetii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
phosphoenolpyruvate + 3-phosphoshikimate	Vibrio cholerae serotype O1	-	phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate	-	r
phosphoenolpyruvate + 3-phosphoshikimate	Coxiella burnetii	-	phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate	-	r
phosphoenolpyruvate + 3-phosphoshikimate	Coxiella burnetii RSA 493	-	phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate	-	r
phosphoenolpyruvate + 3-phosphoshikimate	Vibrio cholerae serotype O1 ATCC 39315	-	phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate	-	r

Organism

Organism	UniProt	Comment	Textmining
Coxiella burnetii	Q83E11	-	-
Coxiella burnetii RSA 493	Q83E11	-	-
Vibrio cholerae serotype O1	Q9KRB0	-	-
Vibrio cholerae serotype O1 ATCC 39315	Q9KRB0	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap nickel affinity column chromatography and HiPrep 26/60 Superdex gel filtration	Vibrio cholerae serotype O1
HisTrap nickel affinity column chromatography and HiPrep 26/60 Superdex gel filtration	Coxiella burnetii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
phosphoenolpyruvate + 3-phosphoshikimate	-	Vibrio cholerae serotype O1	phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate	-	r
phosphoenolpyruvate + 3-phosphoshikimate	-	Coxiella burnetii	phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate	-	r
phosphoenolpyruvate + 3-phosphoshikimate	-	Coxiella burnetii RSA 493	phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate	-	r
phosphoenolpyruvate + 3-phosphoshikimate	-	Vibrio cholerae serotype O1 ATCC 39315	phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate	-	r

Synonyms

Synonyms	Comment	Organism
enolpyruvylshikimate-3-phosphate synthase	-	Vibrio cholerae serotype O1
enolpyruvylshikimate-3-phosphate synthase	-	Coxiella burnetii
EPSPS	-	Vibrio cholerae serotype O1
EPSPS	-	Coxiella burnetii