Literature summary for 2.5.1.19 extracted from

Wang, L.; Peng, R.; Tian, Y.; Han, J.; Zhao, W.; Wang, B.; Liu, M.; Yao, Q.
Characterization of a class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate from Sinorhizobium fredii (2014), World J. Microbiol. Biotechnol., 30, 2967-2973.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Sinorhizobium fredii

Inhibitors

Inhibitors	Comment	Organism	Structure
Al3+	18.3% residual activity at 1 mM	Sinorhizobium fredii
Ca2+	90.1% residual activity at 1 mM	Sinorhizobium fredii
Cu2+	57.6% residual activity at 1 mM	Sinorhizobium fredii
Fe2+	25.2% residual activity at 1 mM	Sinorhizobium fredii
Mn2+	84% residual activity at 1 mM	Sinorhizobium fredii
additional information	insensitive to inhibition by glyphosate exhibiting Ki and IC50 values of 1.2 and 5.2 mM, respectively; not inhibited by EDTA	Sinorhizobium fredii
Zn2+	15.9% residual activity at 1 mM	Sinorhizobium fredii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	194.2% activity at 100 mM	Sinorhizobium fredii
Li+	123.1% activity at 100 mM	Sinorhizobium fredii
Na+	156.6% activity at 100 mM	Sinorhizobium fredii
NH4+	235.8% activity at 100 mM	Sinorhizobium fredii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
47000	-	x * 47000, SDS-PAGE	Sinorhizobium fredii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
phosphoenolpyruvate + 3-phosphoshikimate	Sinorhizobium fredii	-	phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate	-	?
phosphoenolpyruvate + 3-phosphoshikimate	Sinorhizobium fredii NGR234	-	phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate	-	?

Organism

Organism	UniProt	Comment	Textmining
Sinorhizobium fredii	C3MC80	-	-
Sinorhizobium fredii NGR234	C3MC80	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-NTA agarose column chromatography, and gel filtration	Sinorhizobium fredii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + 3-phosphoshikimate	-	Sinorhizobium fredii	phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate	-	?
phosphoenolpyruvate + 3-phosphoshikimate	-	Sinorhizobium fredii NGR234	phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate	-	?

Subunits

Subunits	Comment	Organism
?	x * 47000, SDS-PAGE	Sinorhizobium fredii

Synonyms

Synonyms	Comment	Organism
5-enol-pyruvylshikimate-3-phosphate synthase	-	Sinorhizobium fredii
AroA	-	Sinorhizobium fredii
EPSP synhthase	-	Sinorhizobium fredii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Sinorhizobium fredii

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
40	60	more than 40% activity between 40 and 60°C	Sinorhizobium fredii

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	60	the enzyme has half-lives of 346, 131, 39 and 12 min at 30, 40, 50 and 60°C, respectively	Sinorhizobium fredii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Sinorhizobium fredii

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	9	more than 40% activity between pH 6.0 and 9.0	Sinorhizobium fredii

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	9	the enzyme is stable over a broad range of pH 5.0-9.0, especially at pH 7.5, with more than 80% activity after 1 h incubation	Sinorhizobium fredii

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Release 2023.1 (January 2023)