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Literature summary for 2.5.1.19 extracted from

  • Yi, S.Y.; Cui, Y.; Zhao, Y.; Liu, Z.D.; Lin, Y.J.; Zhou, F.
    A novel naturally occurring class I 5-enolpyruvylshikimate-3-phosphate synthase from Janibacter sp. confers high glyphosate tolerance to rice (2016), Sci. Rep., 6, 19104.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli AB2829 cells Janibacter sp.

Protein Variants

Protein Variants Comment Organism
agriculture the enzyme is efficiently utilized to develop transgenic glyphosate-tolerant crops Janibacter sp.

Inhibitors

Inhibitors Comment Organism Structure
glyphosate
-
Janibacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
phosphoenolpyruvate at pH 8.0 and 40°C Janibacter sp.
0.083
-
3-phosphoshikimate at pH 8.0 and 40°C Janibacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
x * 47000, SDS-PAGE Janibacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate Janibacter sp.
-
phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
?

Organism

Organism UniProt Comment Textmining
Janibacter sp. A3TGI7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate
-
Janibacter sp. phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
?

Subunits

Subunits Comment Organism
? x * 47000, SDS-PAGE Janibacter sp.

Synonyms

Synonyms Comment Organism
5-enol-pyruvylshikimate 3-phosphate synthase
-
Janibacter sp.
AroA
-
Janibacter sp.
EPSPS
-
Janibacter sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.373
-
glyphosate at pH 8.0 and 40°C Janibacter sp.

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.04
-
at pH 8.0 and 40°C Janibacter sp. glyphosate