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Literature summary for 2.5.1.19 extracted from

  • Yu, Q.; Jalaludin, A.; Han, H.; Chen, M.; Sammons, R.D.; Powles, S.B.
    Evolution of a double amino acid substitution in the 5-enolpyruvylshikimate-3-phosphate synthase in Eleusine indica conferring high-level glyphosate resistance (2015), Plant Physiol., 167, 1440-1447.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Eleusine indica

Protein Variants

Protein Variants Comment Organism
P106S the mutant shows high in vitro resistance to glyphosate relative to the wild type enzyme Eleusine indica
T102I/P106S the mutant shows very high-level (2647fold) in vitro resistance to glyphosate relative to the wild type enzyme Eleusine indica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate Eleusine indica
-
 phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?

Organism

Organism UniProt Comment Textmining
Eleusine indica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Eleusine indica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate
-
 Eleusine indica phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?

Synonyms

Synonyms Comment Organism
5-enolpyruvylshikimate-3-phosphate synthase
-
 Eleusine indica
EPSPS
-
 Eleusine indica