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Literature summary for 2.5.1.19 extracted from

  • Herouet-Guicheney, C.; Rouquie, D.; Freyssinet, M.; Currier, T.; Martone, A.; Zhou, J.; Bates, E.E.; Ferullo, J.M.; Hendrickx, K.; Rouan, D.
    Safety evaluation of the double mutant 5-enol pyruvylshikimate-3-phosphate synthase (2mEPSPS) from maize that confers tolerance to glyphosate herbicide in transgenic plants (2009), Regul. Toxicol. Pharmacol., 54, 143-153.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Zea mays

Protein Variants

Protein Variants Comment Organism
T103I/P107S double mutant (47 kDa) which is widely used by genetic engineering to confer glyphosate tolerant properties, to various crops such as corn, cotton, canola, and soybean is assessed in terms of safety aspects: The expressed protein is innocuous. The double mutant enzyme does not possess any of the properties associated with known toxins or allergens, a rapid degradation in simulated gastric and intestinal fluids, and no adverse effects in mice after intravenousor oral administration is shown Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using an anion exchange Q-Sepharose column, ammonium sulfate precipitation using an equilibrated phenyl Sepharose column Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate
-
Zea mays phosphate + 5-enolpyruvylshikimate 3-phosphate
-
?

Synonyms

Synonyms Comment Organism
EPSPS
-
Zea mays