Application | Comment | Organism |
---|---|---|
biotechnology | strain PCC6803 DnaE intein N-terminal and C-terminal splicing domains can reconstitute EPSPS activities and glyphosate resistance in plant | Pseudomonas fluorescens |
Cloned (Comment) | Organism |
---|---|
N-terminal fragment (until Phe295) of G2 EPSPS or the complete gene in pMEB2 (N-terminal of splicing domain of of DnaE intein, strain PCC6803) and C-terminus (from Thr296) of G2 EPSPS in pKEB12 (C-terminal of splicing domain of DnaE intein, strain PCC6803) for intein-mediated protein complementation and expression in Escherichia coli strain ER2799, complete G2 EPSPS gene, N-terminal and C-terminal fragments in pBI121 for introduction into Agrobacterium sp. strain LBA4404 and transformation in Nicotiana tabacum variant NC89 for generation of transgenic plants | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glyphosate | N-phosphonomethylglycine | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0952 | - |
phosphoenolpyruvate | +/-0.00573 mM, full-length G2 EPSPS, crude lysate from overexpressing Escherichia coli ER2799, malachite green dye assay | Pseudomonas fluorescens | |
0.0968 | - |
phosphoenolpyruvate | +/-0.01101 mM, reconstituted G2-EPSPS (by intein-mediated protein complementation), crude lysate from transformed Escherichia coli ER2799, malachite green dye assay | Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20400 | - |
C-terminal fragment (from Thr296) expressed in pKEB12, Western blot | Pseudomonas fluorescens |
47000 | - |
full-length protein, Western blot analyses | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | A1Z0H6 | G2 | - |
Pseudomonas fluorescens G2 | A1Z0H6 | G2 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.48 | - |
+/-0.79 micromol/min/mg, reconstituted G2-EPSPS (by intein-mediated protein complementation), mean specific activity, crude lysate from transformed Escherichia coli ER2799, malachite green dye assay | Pseudomonas fluorescens |
4.761 | - |
+/-0.92 micromol/min/mg, reconstituted G2-EPSPS (by intein-mediated protein complementation), maximum velocity, crude lysate from transformed Escherichia coli ER2799, malachite green dye assay | Pseudomonas fluorescens |
7.12 | - |
+/-0.13 micromol/min/mg, full-length G2 EPSPS, mean specific activity, crude lysate from overexpressing Escherichia coli ER2799, malachite green dye assay | Pseudomonas fluorescens |
7.24 | - |
+/-0.16 micromol/min/mg, full-length G2 EPSPS, maximum velocity, crude lysate from overexpressing Escherichia coli ER2799, malachite green dye assay | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
shikimate 3-phosphate + phosphoenolpyruvate | malachite green dye assay, 28°C | Pseudomonas fluorescens | phosphate + 5-enpolpyruvylshikimate 3-phosphate | - |
? | |
shikimate 3-phosphate + phosphoenolpyruvate | malachite green dye assay, 28°C | Pseudomonas fluorescens G2 | phosphate + 5-enpolpyruvylshikimate 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G2 5-enolpyruvyl shikimate 3-phosphate synthase | glyphosate resistant | Pseudomonas fluorescens |
G2 EPSPS | - |
Pseudomonas fluorescens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
glyphosate | Ki/KM (phosphoenolpyruvate): 0.36, reconstituted G2-EPSPS (by intein-mediated protein complementation), crude lysate from overexpressing Escherichia coli ER2799, malachite green dye assay | Pseudomonas fluorescens | |
additional information | - |
glyphosate | Ki/KM (phosphoenolpyruvate): 0.52, full-length G2 EPSPS, crude lysate from overexpressing Escherichia coli ER2799, malachite green dye assay | Pseudomonas fluorescens | |
0.035 | - |
glyphosate | +/-0.00578 mM, reconstituted G2-EPSPS (by intein-mediated protein complementation), crude lysate from transformed Escherichia coli ER2799, malachite green dye assay | Pseudomonas fluorescens | |
0.0495 | - |
glyphosate | +/-0.00317 mM, full-length G2 EPSPS, crude lysate from overexpressing Escherichia coli ER2799, malachite green dye assay | Pseudomonas fluorescens |