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Literature summary for 2.5.1.19 extracted from

  • Vaithanomsat, P.; Brown, K.A.
    Isolation and mutation of recombinant EPSP synthase from pathogenic bacteria Pseudomonas aeruginosa (2007), Proc. Biochem., 42, 592-598.
No PubMed abstract available
Search for more literature for 2.5.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
G99A KM-value for 5-enolpyruvylshikimate 3-phosphate is 86% of the wild-type value, KM-value for phosphate is 1.3fold higher than wild-type value. Turnover-number for 5-enolpyruvylshikimate 3-phosphate is 6.2fold higher than wild-type value, turnover-number for phosphate is 5.4fold lower than wild-type value Pseudomonas aeruginosa
L104P KM-value for 5-enolpyruvylshikimate 3-phosphate is 1.14fold higher than the wild-type value, KM-value for phosphate is 4.4fold higher than wild-type value. Turnover-number for 5-enolpyruvylshikimate 3-phosphate is 2.7fold lower than wild-type value, turnover-number for phosphate is 1.9fold lower than wild-type value Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
additional information resistant to glyphosate Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0019
-
 5-enolpyruvylshikimate 3-phosphate 25°C, pH 7.0, mutant enzyme G99A Pseudomonas aeruginosa
0.002
-
 5-enolpyruvylshikimate 3-phosphate 25°C, pH 7.0, wild-type enzyme Pseudomonas aeruginosa
0.0025
-
 5-enolpyruvylshikimate 3-phosphate 25°C, pH 7.0, mutant enzyme L104P Pseudomonas aeruginosa
0.0056
-
 phosphate 25°C, pH 7.0, wild-type enzyme Pseudomonas aeruginosa
0.0073
-
 phosphate 25°C, pH 7.0, mutant enzyme G99A Pseudomonas aeruginosa
0.0248
-
 phosphate 25°C, pH 7.0, mutant enzyme L104P Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, SDS-PAGE Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.5
-
 recombinant Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate
-
 Pseudomonas aeruginosa phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 r

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 phosphate 25°C, pH 7.0, mutant enzyme G99A Pseudomonas aeruginosa
1.2
-
 5-enolpyruvylshikimate 3-phosphate 25°C, pH 7.0, mutant enzyme G99A Pseudomonas aeruginosa
2.7
-
 5-enolpyruvylshikimate 3-phosphate 25°C, pH 7.0, mutant strain L104P Pseudomonas aeruginosa
2.8
-
 phosphate 25°C, pH 7.0, mutant enzyme L104P Pseudomonas aeruginosa
5.4
-
 phosphate 25°C, pH 7.0, wild-type enzyme Pseudomonas aeruginosa
7.4
-
 5-enolpyruvylshikimate 3-phosphate 25°C, pH 7.0, wild-type enzyme Pseudomonas aeruginosa