Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli strain BL21(DE3) | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glyphosate | slight inhibition, insensitivity is independent from cations, mechanism | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Staphylococcus aureus | |
0.14 | - |
3-phosphoshikimate | pH 7.0, recombinant enzyme, in presence of 100 mM KCl | Staphylococcus aureus | |
0.18 | - |
phosphoenolpyruvate | pH 7.0, recombinant enzyme, in presence of 100 mM KCl | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slightly activating | Staphylococcus aureus | |
Cs+ | slightly activating | Staphylococcus aureus | |
K+ | highly activating, best at 100 mM | Staphylococcus aureus | |
additional information | no activation by Li+ and Mg2+ | Staphylococcus aureus | |
Na+ | activates | Staphylococcus aureus | |
NH4+ | highly activating | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | Staphylococcus aureus | penultimate step in the shikimate pathway | phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
gene aroA, class II enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | - |
Staphylococcus aureus | phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | penultimate step in the shikimate pathway | Staphylococcus aureus | phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5-enolpyruvylshikimate-3-phosphate synthase | - |
Staphylococcus aureus |
EPSPS | - |
Staphylococcus aureus |
More | class II 5-enolpyruvylshikimate-3-phosphate synthases are less sensitive to glyphosate in contrary to class I 5-enolpyruvylshikimate-3-phosphate synthases which are highly sensitive to inhibition with glyphosate | Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
3-phosphoshikimate | pH 7.0, recombinant enzyme, in presence of 100 mM KCl | Staphylococcus aureus | |
4.3 | - |
phosphoenolpyruvate | pH 7.0, recombinant enzyme, in presence of 100 mM KCl | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Staphylococcus aureus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Staphylococcus aureus |