Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.19 extracted from

  • Priestman, M.A.; Funke, T.; Singh, I.M.; Crupper, S.S.; Schonbrunn, E.
    5-Enolpyruvylshikimate-3-phosphate synthase from Staphylococcus aureus is insensitive to glyphosate (2005), FEBS Lett., 579, 728-732.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli strain BL21(DE3) Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
glyphosate slight inhibition, insensitivity is independent from cations, mechanism Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Staphylococcus aureus
0.14
-
3-phosphoshikimate pH 7.0, recombinant enzyme, in presence of 100 mM KCl Staphylococcus aureus
0.18
-
phosphoenolpyruvate pH 7.0, recombinant enzyme, in presence of 100 mM KCl Staphylococcus aureus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slightly activating Staphylococcus aureus
Cs+ slightly activating Staphylococcus aureus
K+ highly activating, best at 100 mM Staphylococcus aureus
additional information no activation by Li+ and Mg2+ Staphylococcus aureus
Na+ activates Staphylococcus aureus
NH4+ highly activating Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate Staphylococcus aureus penultimate step in the shikimate pathway phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
gene aroA, class II enzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate
-
Staphylococcus aureus phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
?
phosphoenolpyruvate + 3-phosphoshikimate penultimate step in the shikimate pathway Staphylococcus aureus phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
?

Synonyms

Synonyms Comment Organism
5-enolpyruvylshikimate-3-phosphate synthase
-
Staphylococcus aureus
EPSPS
-
Staphylococcus aureus
More class II 5-enolpyruvylshikimate-3-phosphate synthases are less sensitive to glyphosate in contrary to class I 5-enolpyruvylshikimate-3-phosphate synthases which are highly sensitive to inhibition with glyphosate Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2
-
3-phosphoshikimate pH 7.0, recombinant enzyme, in presence of 100 mM KCl Staphylococcus aureus
4.3
-
phosphoenolpyruvate pH 7.0, recombinant enzyme, in presence of 100 mM KCl Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Staphylococcus aureus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information
-
Staphylococcus aureus