Literature summary for 2.5.1.19 extracted from

Oliveira, J.S.; Mendes, M.A.; Palma, M.S.; Basso, L.A.; Santos, D.S.
One-step purification of 5-enolpyruvylshikimate-3-phosphate synthase enzyme from Mycobacterium tuberculosis (2003), Protein Expr. Purif., 28, 287-292.

Search for more literature for 2.5.1.19

Cloned(Commentary)

Cloned (Comment)	Organism
-	Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46425	-	x * 46425, deduced from nucleotide sequence	Mycobacterium tuberculosis
46458	-	x * 46458, electrospray ionization mass spectrometry	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme, Q-Sepharose, one step purification	Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
18.13	-	recombinant enzyme	Mycobacterium tuberculosis

Storage Stability

Storage Stability	Organism
4°C, ammonium sulfate precipitate, 4 months, no loss of activity	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + 3-phosphoshikimate	-	Mycobacterium tuberculosis	phosphate + 5-enolpyruvylshikimate 3-phosphate	-	r

Subunits

Subunits	Comment	Organism
?	x * 46458, electrospray ionization mass spectrometry	Mycobacterium tuberculosis
?	x * 46425, deduced from nucleotide sequence	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)