Cloned (Comment) | Organism |
---|---|
overexpression of wild-type, His-tagged wild-type and several mutant enzymes in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D313A | 0.42% of wild-type activity | Escherichia coli |
D313N | 5% of wild-type activity | Escherichia coli |
D49A | 41% of wild-type activity | Escherichia coli |
E341A | 0.3% of wild-type activity | Escherichia coli |
E341Q | 10% of wild-type activity | Escherichia coli |
H385A | 0.08% of wild-type activity | Escherichia coli |
K22A | 0.7% of wild-type activity | Escherichia coli |
K22R | 3% of wild-type activity | Escherichia coli |
K340A | 2.4% of wild-type activity | Escherichia coli |
K411A | 10.4% of wild-type activity | Escherichia coli |
N94A | 50% of wild-type activity | Escherichia coli |
Q171A | 1.7% of wild-type activity | Escherichia coli |
R100M | 0.2% of wild-type activity | Escherichia coli |
R124A | 19.6% of wild-type activity | Escherichia coli |
R344K | 31.7% of wild-type activity | Escherichia coli |
R344M | 16.3% of wild-type activity | Escherichia coli |
R386M | 15.8% of wild-type activity | Escherichia coli |
Y200F | 1% of wild-type activity | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A6D3 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20 | - |
recombinant His-tagged wild-type enzyme | Escherichia coli |
24 | - |
recombinant wild-type enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | - |
Escherichia coli | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
r |