Cloned (Comment) | Organism |
---|---|
expression of wild-type and G96A mutant enzyme in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
G96A | glyphosate-insensitive | Escherichia coli |
G96A | glyphosate-insensitive | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-phosphonomethylglycine | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.088 | - |
phosphoenolpyruvate | wild-type enzyme | Escherichia coli | |
0.12 | - |
shikimate 3-phosphate | wild-type and G96A mutant enzyme | Escherichia coli | |
2.8 | - |
phosphoenolpyruvate | G96A mutant enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Klebsiella pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and G96A mutant enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | - |
Escherichia coli | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
r | |
phosphoenolpyruvate + 3-phosphoshikimate | - |
Klebsiella pneumoniae | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
r |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
N-phosphonomethylglycine | - |
Escherichia coli |