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Literature summary for 2.5.1.19 extracted from

  • Eschenburg, S.; Healy, M.L.; Priestman, M.A.; Lushington, G.H.; Schonbrunn, E.
    How the mutation glycine96 to alanine confers glyphosate insensitivity to 5-enolpyruvyl shikimate-3-phosphate synthase from Escherichia coli (2002), Planta, 216, 129-135.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and G96A mutant enzyme in Escherichia coli Escherichia coli

Protein Variants

Protein Variants Comment Organism
G96A glyphosate-insensitive Escherichia coli
G96A glyphosate-insensitive Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
N-phosphonomethylglycine
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.088
-
phosphoenolpyruvate wild-type enzyme Escherichia coli
0.12
-
shikimate 3-phosphate wild-type and G96A mutant enzyme Escherichia coli
2.8
-
phosphoenolpyruvate G96A mutant enzyme Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Klebsiella pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and G96A mutant enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate
-
Escherichia coli phosphate + 5-enolpyruvylshikimate 3-phosphate
-
r
phosphoenolpyruvate + 3-phosphoshikimate
-
Klebsiella pneumoniae phosphate + 5-enolpyruvylshikimate 3-phosphate
-
r

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.01
-
N-phosphonomethylglycine
-
Escherichia coli