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Literature summary for 2.5.1.19 extracted from
- The H385N mutant of 5-enolpyruvylshikimate-3-phosphate synthase: kinetics, fluorescence, and nuclear magnetic resonance studies (1996), Arch. Biochem. Biophys., 334, 37-42.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-phosphonomethylglycine | - |
Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
5-enolpyruvylshikimate 3-phosphate | H385N mutant enzyme | Escherichia coli | |
| 0.011 | - |
5-enolpyruvylshikimate 3-phosphate | wild-type enzyme | Escherichia coli | |
| 0.07 | - |
shikimate 3-phosphate | H385 mutant enzyme | Escherichia coli | |
| 0.1 | - |
phosphoenolpyruvate | wild-type enzyme | Escherichia coli | |
| 0.106 | - |
phosphoenolpyruvate | H385N mutant enzyme | Escherichia coli | |
| 0.135 | - |
shikimate 3-phosphate | wild-type enzyme | Escherichia coli | |
| 1.2 | - |
phosphate | H385N mutant enzyme | Escherichia coli | |
| 4.6 | - |
phosphate | wild-type enzyme | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + 3-phosphoshikimate | - |
Escherichia coli | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
H385N mutant enzyme | Escherichia coli |
| 7.8 | - |
wild-type enzyme | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | 8.4 | H385N mutantt enzyme | Escherichia coli |
| 6 | 8.4 | wild-type enzyme | Escherichia coli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0008 | - |
N-phosphonomethylglycine | H385N mutant enzyme | Escherichia coli | |
| 0.0012 | - |
N-phosphonomethylglycine | wild-type enzyme | Escherichia coli | |
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