Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4Cl | maximal activation at 100 mM | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and R24D, P105S, H385L mutant enzymes in Escherichia coli | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
H385L | 0.2% of wild-type activity, 2fold activation at 100 mM NH4Cl | Bacillus subtilis |
H385N | 6% of wild-type activity | Bacillus subtilis |
P105S | 69% of wild-type activity, 8fold activation at 100 mM NH4Cl | Bacillus subtilis |
R24D | 0.8% of wild-type activity, 2fold activation at 100 mM NH4Cl | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-phosphonomethylglycine | 0.25 mM, 50% inhibition of NH4Cl activated enzyme, 75 mM, 50% inhibition of nonactivated enzyme | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
phosphoenolpyruvate | wild-type enzyme | Bacillus subtilis | |
0.16 | - |
shikimate 3-phosphate | H385 mutant enzyme | Bacillus subtilis | |
0.2 | - |
phosphoenolpyruvate | R24D mutant enzyme | Bacillus subtilis | |
0.2 | - |
shikimate 3-phosphate | R24D mutant enzyme | Bacillus subtilis | |
0.33 | - |
shikimate 3-phosphate | wild-type enzyme | Bacillus subtilis | |
0.43 | - |
shikimate 3-phosphate | P105S mutant enzyme | Bacillus subtilis | |
0.59 | - |
phosphoenolpyruvate | P105S mutant enzyme | Bacillus subtilis | |
1 | - |
phosphoenolpyruvate | H385L mutant enzyme | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
mixture of monomers and dimers in the absence of NH4Cl, only monomers in the presence of NH4Cl, gel filtration | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | Bacillus subtilis | involved in aromatic acid biosynthesis | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, ammonium sulfate | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.4 | - |
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | - |
Bacillus subtilis | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r | |
phosphoenolpyruvate + 3-phosphoshikimate | involved in aromatic acid biosynthesis | Bacillus subtilis | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
N-phosphonomethylglycine | H385L mutant enzyme | Bacillus subtilis | |
0.05 | - |
N-phosphonomethylglycine | R24D mutant enzyme | Bacillus subtilis | |
0.06 | - |
N-phosphonomethylglycine | wild-type enzyme | Bacillus subtilis | |
0.21 | - |
N-phosphonomethylglycine | P105S mutant enzyme | Bacillus subtilis |