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Literature summary for 2.5.1.19 extracted from
- 5-enol-Pyruvyl-shikimate-3-phosphate synthase from Zea Mays cultured cells. Purification and properties (1994), Plant Physiol., 105, 1107-1114.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-phosphonomethylglycine | 0.006-0.008 mM, 50% inhibition, 1 mM, complete inhibition | Zea mays |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.028 | - |
phosphoenolpyruvate | isoenzyme II | Zea mays | |
| 0.029 | - |
shikimate 3-phosphate | isoenzyme II | Zea mays | |
| 0.033 | - |
phosphoenolpyruvate | isoenzyme I | Zea mays | |
| 0.033 | - |
shikimate 3-phosphate | isoenzyme I | Zea mays | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | isoenzymes I and II | Zea mays | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53600 | - |
1 * 53600, isoenzyme II, SDS-PAGE | Zea mays |
| 54900 | - |
1 * 54900, isoenzyme I, SDS-PAGE | Zea mays |
| 58100 | - |
isoenzyme II, gel filtration | Zea mays |
| 58700 | - |
isoenzyme I, gel filtration | Zea mays |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| no activity in mammals | - |
- |
- |
| Petunia x hybrida | - |
- |
- |
| Zea mays | - |
var. Black Mexican Sweet, isoenzymes I and II | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-Sephacel, cellulose phosphate column, isoenzymes I and II | Zea mays |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 61 | - |
isoenzyme I | Zea mays |
| 94.7 | - |
isoenzyme II | Zea mays |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 3 months, 15% loss of activity | Zea mays |
| 0°C, 30 d, 25% loss of activity | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + 3-phosphoshikimate | - |
Zea mays | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r | |
| phosphoenolpyruvate + 3-phosphoshikimate | - |
Petunia x hybrida | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 53600, isoenzyme II, SDS-PAGE | Zea mays |
| monomer | 1 * 54900, isoenzyme I, SDS-PAGE | Zea mays |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
isoenzymes I and II | Zea mays |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
isoenzyme I, 50% loss of activity after 20 min, isoenzyme II, 50% loss of activity after 60 min | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 7.5 | isoenzymes I and II | Zea mays |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00012 | 0.00013 | N-phosphonomethylglycine | isoenzymes I and II, vs. phosphoenolpyruvate | Zea mays | |
| 0.0094 | - |
N-phosphonomethylglycine | isoenzyme I, vs. shikimate 3-phosphate | Zea mays | |
| 0.012 | - |
N-phosphonomethylglycine | isoenzyme II, vs. shikimate 3-phosphate | Zea mays | |
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