Literature summary for 2.5.1.19 extracted from

Rubin, J.L.; Gaines, C.G.; Jensen, R.A.
Glyphosphate inhibition of 5-enolpyruvylshikimate 3-phosphate synthase from suspension-cultured cells of Nicotiana silvestris (1984), Plant Physiol., 75, 839-845.

Search for more literature for 2.5.1.19

Inhibitors

Inhibitors	Comment	Organism	Structure
N-phosphonomethylglycine	0.0183 mM, 50% inhibition, 0.5 mM 96% inhibition, competitive vs. phosphoenolpyruvate, uncompetitive vs. shikimate 3-phosphate at pH 7.0, mechanism; pH-dependent	Nicotiana sylvestris

Organism

Organism	UniProt	Comment	Textmining
Nicotiana sylvestris	-	cv. Speg et Comes, aneuploid cell line ANS-1	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-cellulose, CM-cellulose, partial purification	Nicotiana sylvestris

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell suspension culture	-	Nicotiana sylvestris	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
16.9	-	-	Nicotiana sylvestris

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + 3-phosphoshikimate	-	Nicotiana sylvestris	phosphate + 5-enolpyruvylshikimate 3-phosphate	i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate	r

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	7.2	50% of maximal activity between pH 6.2 and pH 8.5	Nicotiana sylvestris

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.2	8.5	approx. half-maximal activity at pH 6.2 and 8.5	Nicotiana sylvestris

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00125	-	N-phosphonomethylglycine	vs. phosphoenolpyruvate	Nicotiana sylvestris
0.0183	-	N-phosphonomethylglycine	vs. shikimate 3-phosphate	Nicotiana sylvestris

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Release 2023.1 (January 2023)