General Stability | Organism |
---|---|
glyphosate-sensitive enzyme is more stable than wild-type enzyme | Klebsiella pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Bromopyruvate | 1 mM, 50% inactivation after 3.4 min | Klebsiella pneumoniae | |
N-ethylmaleimide | 1 mM, 50% inactivation after approx. 3 min | Klebsiella pneumoniae | |
N-phosphonomethylglycine | wild-type enzyme | Klebsiella pneumoniae | |
Phenylglyoxal | 2 mM, 50% inactivation after 32 min | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
phosphoenolpyruvate | glyphosate-sensitive enzyme | Klebsiella pneumoniae | |
0.045 | - |
3-phosphoshikimate | glyphosate-sensitive enzyme | Klebsiella pneumoniae | |
0.14 | - |
phosphoenolpyruvate | glyphosate-resistant enzyme | Klebsiella pneumoniae | |
0.193 | - |
3-phosphoshikimate | glyphosate-resistant enzyme | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42500 | - |
1 * 42500, wild-type enzyme, SDS-PAGE | Klebsiella pneumoniae |
42900 | - |
1 * 42900, glyphosate-resistant enzyme, SDS-PAGE | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
formerly Aerobacter aerogenes, glyphosate sensitive and resistant strains | - |
Purification (Comment) | Organism |
---|---|
glyphosate insensitve strain, ammonium sulfate, heat treatment, DEAE-cellulose, Sephadex G-75, cellulose phosphate, chromatofocusing | Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.86 | - |
glyphosate insensitve strain | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | - |
Klebsiella pneumoniae | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42500, wild-type enzyme, SDS-PAGE | Klebsiella pneumoniae |
monomer | 1 * 42900, glyphosate-resistant enzyme, SDS-PAGE | Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Klebsiella pneumoniae |
60 | - |
wild-type enzyme | Klebsiella pneumoniae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
rapid inactivation above, glyphosate resistant enzyme | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 4.1, glyphosate-resistant enzyme | Klebsiella pneumoniae |
additional information | - |
pI: 4.6, wild-type enzyme | Klebsiella pneumoniae |
5.4 | - |
wild-type enzyme, 2 maxima at pH 5.4 and pH 7.0 | Klebsiella pneumoniae |
6 | - |
glyphosate-resistant enzyme, 2 maxima at pH 6 and pH 7.3 | Klebsiella pneumoniae |
7 | - |
wild-type enzyme, 2 maxima at pH 5.4 and pH 7.0 | Klebsiella pneumoniae |
7.3 | - |
glyphosate-resistant enzyme, 2 maxima at pH 6 and pH 7.3 | Klebsiella pneumoniae |