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Literature summary for 2.5.1.19 extracted from

  • Sost, D.; Chulz, A.; Amrhein, N.
    Characterizaation of a glyphosphate-insensitive 5-enolpyruvylshikimic acid-3-phosphate synthase (1984), FEBS Lett., 173, 238-242.
No PubMed abstract available

General Stability

General Stability Organism
glyphosate-sensitive enzyme is more stable than wild-type enzyme Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
3-Bromopyruvate 1 mM, 50% inactivation after 3.4 min Klebsiella pneumoniae
N-ethylmaleimide 1 mM, 50% inactivation after approx. 3 min Klebsiella pneumoniae
N-phosphonomethylglycine wild-type enzyme Klebsiella pneumoniae
Phenylglyoxal 2 mM, 50% inactivation after 32 min Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
phosphoenolpyruvate glyphosate-sensitive enzyme Klebsiella pneumoniae
0.045
-
3-phosphoshikimate glyphosate-sensitive enzyme Klebsiella pneumoniae
0.14
-
phosphoenolpyruvate glyphosate-resistant enzyme Klebsiella pneumoniae
0.193
-
3-phosphoshikimate glyphosate-resistant enzyme Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42500
-
1 * 42500, wild-type enzyme, SDS-PAGE Klebsiella pneumoniae
42900
-
1 * 42900, glyphosate-resistant enzyme, SDS-PAGE Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
formerly Aerobacter aerogenes, glyphosate sensitive and resistant strains
-

Purification (Commentary)

Purification (Comment) Organism
glyphosate insensitve strain, ammonium sulfate, heat treatment, DEAE-cellulose, Sephadex G-75, cellulose phosphate, chromatofocusing Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.86
-
glyphosate insensitve strain Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate
-
Klebsiella pneumoniae phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r

Subunits

Subunits Comment Organism
monomer 1 * 42500, wild-type enzyme, SDS-PAGE Klebsiella pneumoniae
monomer 1 * 42900, glyphosate-resistant enzyme, SDS-PAGE Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Klebsiella pneumoniae
60
-
wild-type enzyme Klebsiella pneumoniae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
rapid inactivation above, glyphosate resistant enzyme Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pI: 4.1, glyphosate-resistant enzyme Klebsiella pneumoniae
additional information
-
pI: 4.6, wild-type enzyme Klebsiella pneumoniae
5.4
-
wild-type enzyme, 2 maxima at pH 5.4 and pH 7.0 Klebsiella pneumoniae
6
-
glyphosate-resistant enzyme, 2 maxima at pH 6 and pH 7.3 Klebsiella pneumoniae
7
-
wild-type enzyme, 2 maxima at pH 5.4 and pH 7.0 Klebsiella pneumoniae
7.3
-
glyphosate-resistant enzyme, 2 maxima at pH 6 and pH 7.3 Klebsiella pneumoniae