Cloned (Comment) | Organism |
---|---|
Escherichia coli structural gene aroA | Escherichia coli |
subcloned from bacteriophage lambdapserC into multicopy plasmid pAT153 with subsequent transformation of Escherichia coli AB2829 CGSC2829 cells | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-enolpyruvylshikimate 3-phosphate | product inhibition | Escherichia coli | |
N-phosphonomethylglycine | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
3-phosphoshikimate | recombinant overproducing strain | Escherichia coli | |
0.003 | - |
5-enolpyruvylshikimate 3-phosphate | recombinant overproducing strain | Escherichia coli | |
0.0032 | 0.0036 | 3-phosphoshikimate | wild-type enzyme | Escherichia coli | |
0.015 | - |
phosphoenolpyruvate | wild-type enzyme | Escherichia coli | |
0.016 | - |
phosphoenolpyruvate | recombinant overproducing strain | Escherichia coli | |
2.5 | - |
phosphate | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | Escherichia coli | involved in aromatic acid biosynthesis | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
overproducing recombinant strain AB2829pKD501 | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.1 | - |
phosphate + 5-enolpyruvylshikimate 3-phosphate, recombinant enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | - |
Escherichia coli | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r | |
phosphoenolpyruvate + 3-phosphoshikimate | involved in aromatic acid biosynthesis | Escherichia coli | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
N-phosphonomethylglycine | vs. shikimate 3-phosphate, recombinant enzyme | Escherichia coli | |
0.001 | - |
N-phosphonomethylglycine | vs. shikimate 3-phosphate, native enzyme | Escherichia coli |