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Literature summary for 2.5.1.19 extracted from

  • Duncan, K.; Lewendon, A.; Coggins, J.R.
    The purification of 5-enolpyruvylshikimate 3-phosphate synthase from an overproducing strain of Escherichia coli (1984), FEBS Lett., 165, 121-127.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli structural gene aroA Escherichia coli
subcloned from bacteriophage lambdapserC into multicopy plasmid pAT153 with subsequent transformation of Escherichia coli AB2829 CGSC2829 cells Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
5-enolpyruvylshikimate 3-phosphate product inhibition Escherichia coli
N-phosphonomethylglycine
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
3-phosphoshikimate recombinant overproducing strain Escherichia coli
0.003
-
5-enolpyruvylshikimate 3-phosphate recombinant overproducing strain Escherichia coli
0.0032 0.0036 3-phosphoshikimate wild-type enzyme Escherichia coli
0.015
-
phosphoenolpyruvate wild-type enzyme Escherichia coli
0.016
-
phosphoenolpyruvate recombinant overproducing strain Escherichia coli
2.5
-
phosphate
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate Escherichia coli involved in aromatic acid biosynthesis phosphate + 5-enolpyruvylshikimate 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
overproducing recombinant strain AB2829pKD501
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.1
-
phosphate + 5-enolpyruvylshikimate 3-phosphate, recombinant enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + 3-phosphoshikimate
-
Escherichia coli phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r
phosphoenolpyruvate + 3-phosphoshikimate involved in aromatic acid biosynthesis Escherichia coli phosphate + 5-enolpyruvylshikimate 3-phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0009
-
N-phosphonomethylglycine vs. shikimate 3-phosphate, recombinant enzyme Escherichia coli
0.001
-
N-phosphonomethylglycine vs. shikimate 3-phosphate, native enzyme Escherichia coli