Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Danio rerio |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 90% residual activity at 5 mM | Danio rerio | |
Cd2+ | complete inhibition at 5 mM | Danio rerio | |
Cu2+ | complete inhibition at 5 mM | Danio rerio | |
Fe2+ | about 30% residual activity at 5 mM | Danio rerio | |
Hg2+ | complete inhibition at 5 mM | Danio rerio | |
Mg2+ | about 81% residual activity at 5 mM | Danio rerio | |
Mn2+ | about 95% residual activity at 5 mM | Danio rerio | |
Pb2+ | complete inhibition at 5 mM | Danio rerio | |
Zn2+ | about 1% residual activity at 5 mM | Danio rerio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.81 | - |
glutathione | at pH 7.5 and 25°C | Danio rerio | |
6.76 | - |
1-chloro-2,4-dinitrobenzene | at pH 7.5 and 25°C | Danio rerio |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Danio rerio | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | slight stimulation of activity | Danio rerio |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | Q9DDU5 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose column chromatography | Danio rerio |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.04483 | - |
with 1-chloro-2,4-dinitrobenzene as substrate, at pH 7.5 and 25°C | Danio rerio |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Danio rerio | chloride + 2,4-dinitrophenyl-glutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, SDS-PAGE | Danio rerio |
? | x * 23530, calculated from amino acid sequence | Danio rerio |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase Pi-1 | - |
Danio rerio |
GSTP1 | - |
Danio rerio |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Danio rerio |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | the enzyme is active between pH 6.0 and 8.0 | Danio rerio |