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Literature summary for 2.5.1.18 extracted from
- Identification and characterization of the zebrafish glutathione S-transferase Pi-1 (2017), J. Biochem. Mol. Toxicol., 31, e21948 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Danio rerio |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | about 90% residual activity at 5 mM | Danio rerio |  |
| Cd2+ | complete inhibition at 5 mM | Danio rerio | |
| Cu2+ | complete inhibition at 5 mM | Danio rerio | |
| Fe2+ | about 30% residual activity at 5 mM | Danio rerio | |
| Hg2+ | complete inhibition at 5 mM | Danio rerio | |
| Mg2+ | about 81% residual activity at 5 mM | Danio rerio | |
| Mn2+ | about 95% residual activity at 5 mM | Danio rerio | |
| Pb2+ | complete inhibition at 5 mM | Danio rerio | |
| Zn2+ | about 1% residual activity at 5 mM | Danio rerio | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.81 | - |
glutathione | at pH 7.5 and 25°C | Danio rerio | |
| 6.76 | - |
1-chloro-2,4-dinitrobenzene | at pH 7.5 and 25°C | Danio rerio | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Danio rerio | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | slight stimulation of activity | Danio rerio | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Danio rerio | Q9DDU5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione-Sepharose column chromatography | Danio rerio |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.04483 | - |
with 1-chloro-2,4-dinitrobenzene as substrate, at pH 7.5 and 25°C | Danio rerio |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Danio rerio | chloride + 2,4-dinitrophenyl-glutathione | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25000, SDS-PAGE | Danio rerio |
| ? | x * 23530, calculated from amino acid sequence | Danio rerio |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione S-transferase Pi-1 | - |
Danio rerio |
| GSTP1 | - |
Danio rerio |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Danio rerio |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | the enzyme is active between pH 6.0 and 8.0 | Danio rerio |
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