Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.18 extracted from

  • Gonzalez, D.; Fraichard, S.; Grassein, P.; Delarue, P.; Senet, P.; Nicolai, A.; Chavanne, E.; Mucher, E.; Artur, Y.; Ferveur, J.F.; Heydel, J.M.; Briand, L.; Neiers, F.
    Characterization of a Drosophila glutathione transferase involved in isothiocyanate detoxification (2018), Insect Biochem. Mol. Biol., 95, 33-43 .
    View publication on PubMed
Search for more literature for 2.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Silex cells Drosophila melanogaster

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1M succinic acid-phosphate-glycine buffer with 25% (w/v) PEG 1500 at pH 4.0 Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
allyl-isothiocyanate about 30% inhibition at 0.01 mM Drosophila melanogaster
Berberine complete inhibition at 0.1 mM Drosophila melanogaster
Caffeine about 25% inhibition at 0.01 mM Drosophila melanogaster
chrysin about 30% inhibition at 0.1 mM Drosophila melanogaster
coumarin about 17% inhibition at 0.01 mM Drosophila melanogaster
denatonium benzoate about 10% inhibition at 0. 1 mM Drosophila melanogaster
Digitonin about 20% inhibition at 0.01 mM Drosophila melanogaster
escin about 10% inhibition at 0.1 mM Drosophila melanogaster
fisetin 60% inhibition at 0.1 mM Drosophila melanogaster
flavonol about 20% inhibition at 0.01 mM Drosophila melanogaster
kaempferol about 42% inhibition at 0.01 mM Drosophila melanogaster
luteolin about 16% inhibition at 0.01 mM Drosophila melanogaster
naringenin 42% inhibition at 0.1 mM Drosophila melanogaster
naringin about 17% inhibition at 0.01 mM Drosophila melanogaster
Nicotine about 16% inhibition at 0.01 mM Drosophila melanogaster
papaverine about 5% inhibition at 0.1 mM Drosophila melanogaster
paraxanthine about 9% inhibition at 0.01 mM Drosophila melanogaster
phenethyl-isothiocyanate about 25% inhibition at 0.01 mM Drosophila melanogaster
piperidine about 15% inhibition at 0.1 mM Drosophila melanogaster
propyl-isothiocyanate 42% inhibition at 0.01 mM Drosophila melanogaster
Pyridine about 17% inhibition at 0.1 mM Drosophila melanogaster
quercetin complete inhibition at 0.1 mM, 52% inhibition at 0.01 mM Drosophila melanogaster
Quinine about 18% inhibition at 0.1 mM Drosophila melanogaster
sinigrin hydrate about 28% inhibition at 0.01 mM Drosophila melanogaster
strychnine about 37% inhibition at 0.01 mM Drosophila melanogaster
tangeretin complete inhibition at 0.1 mM Drosophila melanogaster
taurine about 19% inhibition at 0.1 mM Drosophila melanogaster
Theobromine about 12% inhibition at 0.01 mM Drosophila melanogaster
theophylline about 10% inhibition at 0.01 mM Drosophila melanogaster
thiamine hydrochloride about 5% inhibition at 0.1 mM Drosophila melanogaster
thiazolidine about 18% inhibition at 0.01 mM Drosophila melanogaster

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49850
-
 SEC-MALS analysis Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q9VG98
-
-

Purification (Commentary)

Purification (Comment) Organism
GSTrap column chromatography Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
antenna
-
 Drosophila melanogaster
-
foreleg
-
 Drosophila melanogaster
-
head
-
 Drosophila melanogaster
-
maxillary palp
-
 Drosophila melanogaster
-
olfactory organ
-
 Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8
-
 with propyl-isothiocyanate as substrate, at pH 6.4, temperature not specified in the publication Drosophila melanogaster
36
-
 with allyl-isothiocyanate as substrate, at pH 6.4, temperature not specified in the publication Drosophila melanogaster
74
-
 with phenethyl-isothiocyanate as substrate, at pH 6.4, temperature not specified in the publication Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
-
 Drosophila melanogaster chloride + 2,4-dinitrophenyl-glutathione
-
 ?
glutathione + allyl-isothiocyanate
-
 Drosophila melanogaster ?
-
 ?
glutathione + phenethyl-isothiocyanate
-
 Drosophila melanogaster ?
-
 ?
glutathione + propyl-isothiocyanate
-
 Drosophila melanogaster ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 25000, SDS-PAGE Drosophila melanogaster
homodimer 2 * 24535, calculated from amino acid sequence Drosophila melanogaster

Synonyms

Synonyms Comment Organism
GST Delta 2 isoform Drosophila melanogaster
GSTD2 isoform Drosophila melanogaster

Expression

Organism Comment Expression
Drosophila melanogaster in the olfactory organs, the GSTD2 mRNA level is strongly decreased (5.9fold) in the presence of phenethyl-isothiocyanate down
Drosophila melanogaster isoform GSTD2 expression levels increase in the presence of propyl-, phenethyl- and allyl-isothiocyanates with phenethyl-isothiocyanate (0.25 mM) inducing the highest effect (6fold increase). Phenethyl-isothiocyanate highly induces GSTD2 mRNA expression in the taste organs (3.5fold) up

General Information

General Information Comment Organism
physiological function the enzyme plays a biological role in chemoperception Drosophila melanogaster