Literature summary for 2.5.1.18 extracted from

Zhuge, X.L.; Xu, H.; Xiu, Z.J.; Yang, H.L.
Biochemical functions of glutathione S-transferase family of Salix babylonica (2020), Front. Plant Sci., 11, 364 .

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) cells	Salix babylonica

Protein Variants

Protein Variants	Comment	Organism
I55T	the mutant of isoform GSTU6 shows strongly decreased activity compared to the wild type enzyme	Salix babylonica
T53I	the mutant of isoform GSTU7 shows strongly increased activity compared to the wild type enzyme	Salix babylonica
T53V	the mutant of isoform GSTU7 shows strongly increased activity compared to the wild type enzyme	Salix babylonica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.14	-	glutathione	isoform GSTU3, at pH 6.5 and 25°C	Salix babylonica
0.141	-	glutathione	isoform GSTU6, at pH 6.5 and 25°C	Salix babylonica
0.146	-	glutathione	isoform GSTU4, at pH 6.5 and 25°C	Salix babylonica
0.156	-	1-chloro-2,4-dinitrobenzene	isoform GSTU10, at pH 6.5 and 25°C	Salix babylonica
0.201	-	1-chloro-2,4-dinitrobenzene	isoform GSTU1, at pH 6.5 and 25°C	Salix babylonica
0.208	-	glutathione	isoform GSTU9, at pH 6.5 and 25°C	Salix babylonica
0.24	-	glutathione	isoform GSTU5, at pH 6.5 and 25°C	Salix babylonica
0.262	-	glutathione	isoform GSTU13, at pH 6.5 and 25°C	Salix babylonica
0.284	-	glutathione	isoform GSTU10, at pH 6.5 and 25°C	Salix babylonica
0.333	-	glutathione	isoform GSTU12, at pH 6.5 and 25°C	Salix babylonica
0.473	-	glutathione	isoform GSTU1, at pH 6.5 and 25°C	Salix babylonica
0.491	-	glutathione	isoform GSTU14, at pH 6.5 and 25°C	Salix babylonica
0.732	-	glutathione	isoform GSTU11, at pH 6.5 and 25°C	Salix babylonica
0.893	-	glutathione	isoform GSTU8, at pH 6.5 and 25°C	Salix babylonica
0.996	-	glutathione	isoform GSTU2, at pH 6.5 and 25°C	Salix babylonica
1.003	-	1-chloro-2,4-dinitrobenzene	isoform GSTU3, at pH 6.5 and 25°C	Salix babylonica
1.183	-	glutathione	isoform GSTU7, at pH 6.5 and 25°C	Salix babylonica
1.273	-	1-chloro-2,4-dinitrobenzene	isoform GSTU13, at pH 6.5 and 25°C	Salix babylonica
2.074	-	1-chloro-2,4-dinitrobenzene	isoform GSTU7, at pH 6.5 and 25°C	Salix babylonica
2.144	-	1-chloro-2,4-dinitrobenzene	isoform GSTU12, at pH 6.5 and 25°C	Salix babylonica
2.266	-	1-chloro-2,4-dinitrobenzene	isoform GSTU6, at pH 6.5 and 25°C	Salix babylonica
2.364	-	1-chloro-2,4-dinitrobenzene	isoform GSTU5, at pH 6.5 and 25°C	Salix babylonica
2.628	-	1-chloro-2,4-dinitrobenzene	isoform GSTU8, at pH 6.5 and 25°C	Salix babylonica
2.785	-	1-chloro-2,4-dinitrobenzene	isoform GSTU9, at pH 6.5 and 25°C	Salix babylonica
3.372	-	1-chloro-2,4-dinitrobenzene	isoform GSTU11, at pH 6.5 and 25°C	Salix babylonica
4.269	-	1-chloro-2,4-dinitrobenzene	isoform GSTU14, at pH 6.5 and 25°C	Salix babylonica
4.819	-	1-chloro-2,4-dinitrobenzene	isoform GSTU2, at pH 6.5 and 25°C	Salix babylonica
5.121	-	1-chloro-2,4-dinitrobenzene	isoform GSTU4, at pH 6.5 and 25°C	Salix babylonica

Organism

Organism	UniProt	Comment	Textmining
Salix babylonica	A0A4Y5QZW9	-	-
Salix babylonica	A0A4Y5QZX0	-	-
Salix babylonica	A0A4Y5QZX2	-	-
Salix babylonica	A0A4Y5QZX3	-	-
Salix babylonica	A0A4Y5QZX9	-	-
Salix babylonica	A0A4Y5QZZ8	-	-
Salix babylonica	A0A4Y5R032	-	-
Salix babylonica	A0A4Y5R087	-	-
Salix babylonica	A0A4Y5R0B2	-	-
Salix babylonica	A0A4Y5R0C1	-	-
Salix babylonica	A0A4Y5R0K8	-	-
Salix babylonica	A0A4Y5R0L5	-	-
Salix babylonica	A0A4Y5R2M7	-	-
Salix babylonica	A0A4Y5R2N2	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel-Sepharose column chromatography	Salix babylonica

Source Tissue

Source Tissue	Comment	Organism	Textmining
bud	-	Salix babylonica	-
flower	-	Salix babylonica	-
flower	highest expression	Salix babylonica	-
flower	high expression	Salix babylonica	-
leaf	-	Salix babylonica	-
leaf	highest expression	Salix babylonica	-
phloem	-	Salix babylonica	-
phloem	high expression	Salix babylonica	-
root	-	Salix babylonica	-
root	highest expression	Salix babylonica	-
root	high expression	Salix babylonica	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
glutathione + 1,2-dichloro-4-nitrobenzene	-	Salix babylonica	?	-	?
glutathione + 1-chloro-2,4-dinitrobenzene	-	Salix babylonica	chloride + 2,4-dinitrophenyl-glutathione	-	?
glutathione + 4-nitrobenzyl chloride	-	Salix babylonica	chloride + 4-nitrobenzyl-glutathione	-	?
glutathione + 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole	-	Salix babylonica	?	-	?
glutathione + cumene hydroperoxide	-	Salix babylonica	?	-	?
glutathione + fluorodifen	-	Salix babylonica	?	-	?
additional information	no activity with 1,2-dichloro-4-nitrobenzene, dehydroascorbic acid, 4-nitrobenzyl chloride and cumene hydroperoxide	Salix babylonica	?	-	-
additional information	no activity with dehydroascorbic acid and cumene hydroperoxide	Salix babylonica	?	-	-
additional information	no activity with dehydroascorbic acid, 1,2-dichloro-4-nitrobenzene and cumene hydroperoxide	Salix babylonica	?	-	-
additional information	no activity with dehydroascorbic acid, 4-nitrobenzyl chloride and cumene hydroperoxide	Salix babylonica	?	-	-
additional information	no activity with dehydroascorbic acid, 4-nitrobenzyl chloride, 1,2-dichloro-4-nitrobenzene and cumene hydroperoxide	Salix babylonica	?	-	-
additional information	no activity with fluorodifen, 1,2-dichloro-4-nitrobenzene, dehydroascorbic acid, 4-nitrobenzyl chloride and cumene hydroperoxide	Salix babylonica	?	-	-

Subunits

Subunits	Comment	Organism
?	x * 26100, calculated from amino acid sequence	Salix babylonica
?	x * 24756, calculated from amino acid sequence	Salix babylonica
?	x * 24893, calculated from amino acid sequence	Salix babylonica
?	x * 25014, calculated from amino acid sequence	Salix babylonica
?	x * 25172, calculated from amino acid sequence	Salix babylonica
?	x * 25262, calculated from amino acid sequence	Salix babylonica
?	x * 25300, calculated from amino acid sequence	Salix babylonica
?	x * 25340, calculated from amino acid sequence	Salix babylonica
?	x * 25401, calculated from amino acid sequence	Salix babylonica
?	x * 25493, calculated from amino acid sequence	Salix babylonica
?	x * 25536, calculated from amino acid sequence	Salix babylonica
?	x * 25567, calculated from amino acid sequence	Salix babylonica
?	x * 25680, calculated from amino acid sequence	Salix babylonica
?	x * 26434, calculated from amino acid sequence	Salix babylonica

Synonyms

Synonyms	Comment	Organism
glutathione S-transferase	-	Salix babylonica
GST	-	Salix babylonica
GSTU1	isoform	Salix babylonica
GSTU10	isoform	Salix babylonica
GSTU11	isoform	Salix babylonica
GSTU12	isoform	Salix babylonica
GSTU13	isoform	Salix babylonica
GSTU14	isoform	Salix babylonica
GSTU2	isoform	Salix babylonica
GSTU3	isoform	Salix babylonica
GSTU4	isoform	Salix babylonica
GSTU5	isoform	Salix babylonica
GSTU6	isoform	Salix babylonica
GSTU7	isoform	Salix babylonica
GSTU8	isoform	Salix babylonica
GSTU9	isoform	Salix babylonica

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.804	-	glutathione	isoform GSTU7, at pH 6.5 and 25°C	Salix babylonica
1.197	-	glutathione	isoform GSTU8, at pH 6.5 and 25°C	Salix babylonica
1.314	-	glutathione	isoform GSTU12, at pH 6.5 and 25°C	Salix babylonica
1.415	-	1-chloro-2,4-dinitrobenzene	isoform GSTU7, at pH 6.5 and 25°C	Salix babylonica
1.934	-	1-chloro-2,4-dinitrobenzene	isoform GSTU12, at pH 6.5 and 25°C	Salix babylonica
2.061	-	1-chloro-2,4-dinitrobenzene	isoform GSTU8, at pH 6.5 and 25°C	Salix babylonica
2.496	-	glutathione	isoform GSTU2, at pH 6.5 and 25°C	Salix babylonica
2.963	-	glutathione	isoform GSTU4, at pH 6.5 and 25°C	Salix babylonica
3.376	-	glutathione	isoform GSTU5, at pH 6.5 and 25°C	Salix babylonica
6.056	-	1-chloro-2,4-dinitrobenzene	isoform GSTU1, at pH 6.5 and 25°C	Salix babylonica
6.283	-	glutathione	isoform GSTU1, at pH 6.5 and 25°C	Salix babylonica
7.391	-	glutathione	isoform GSTU10, at pH 6.5 and 25°C	Salix babylonica
7.564	-	glutathione	isoform GSTU9, at pH 6.5 and 25°C	Salix babylonica
7.782	-	1-chloro-2,4-dinitrobenzene	isoform GSTU5, at pH 6.5 and 25°C	Salix babylonica
11.4	-	glutathione	isoform GSTU13, at pH 6.5 and 25°C	Salix babylonica
12.281	-	glutathione	isoform GSTU14, at pH 6.5 and 25°C	Salix babylonica
14.948	-	1-chloro-2,4-dinitrobenzene	isoform GSTU13, at pH 6.5 and 25°C	Salix babylonica
16.183	-	1-chloro-2,4-dinitrobenzene	isoform GSTU10, at pH 6.5 and 25°C	Salix babylonica
16.302	-	glutathione	isoform GSTU11, at pH 6.5 and 25°C	Salix babylonica
16.483	-	glutathione	isoform GSTU6, at pH 6.5 and 25°C	Salix babylonica
16.714	-	glutathione	isoform GSTU3, at pH 6.5 and 25°C	Salix babylonica
21.06	-	1-chloro-2,4-dinitrobenzene	isoform GSTU9, at pH 6.5 and 25°C	Salix babylonica
22.472	-	1-chloro-2,4-dinitrobenzene	isoform GSTU11, at pH 6.5 and 25°C	Salix babylonica
24.482	-	1-chloro-2,4-dinitrobenzene	isoform GSTU4, at pH 6.5 and 25°C	Salix babylonica
28.819	-	1-chloro-2,4-dinitrobenzene	isoform GSTU3, at pH 6.5 and 25°C	Salix babylonica
41.823	-	1-chloro-2,4-dinitrobenzene	isoform GSTU14, at pH 6.5 and 25°C	Salix babylonica
112.987	-	1-chloro-2,4-dinitrobenzene	isoform GSTU6, at pH 6.5 and 25°C	Salix babylonica

pI Value

Organism	Comment	pI Value Maximum	pI Value
Salix babylonica	calculated from amino acid sequence	-	5.1
Salix babylonica	calculated from amino acid sequence	-	5.2
Salix babylonica	calculated from amino acid sequence	-	5.3
Salix babylonica	calculated from amino acid sequence	-	5.5
Salix babylonica	calculated from amino acid sequence	-	5.8
Salix babylonica	calculated from amino acid sequence	-	5.9
Salix babylonica	calculated from amino acid sequence	-	6.2
Salix babylonica	calculated from amino acid sequence	-	6.4
Salix babylonica	calculated from amino acid sequence	-	6.6
Salix babylonica	calculated from amino acid sequence	-	6.9

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
0.679	-	glutathione	isoform GSTU7, at pH 6.5 and 25°C	Salix babylonica
0.682	-	1-chloro-2,4-dinitrobenzene	isoform GSTU7, at pH 6.5 and 25°C	Salix babylonica
0.784	-	1-chloro-2,4-dinitrobenzene	isoform GSTU8, at pH 6.5 and 25°C	Salix babylonica
0.902	-	1-chloro-2,4-dinitrobenzene	isoform GSTU12, at pH 6.5 and 25°C	Salix babylonica
1.325	-	1-chloro-2,4-dinitrobenzene	isoform GSTU2, at pH 6.5 and 25°C	Salix babylonica
1.34	-	glutathione	isoform GSTU8, at pH 6.5 and 25°C	Salix babylonica
2.506	-	glutathione	isoform GSTU2, at pH 6.5 and 25°C	Salix babylonica
3.292	-	1-chloro-2,4-dinitrobenzene	isoform GSTU5, at pH 6.5 and 25°C	Salix babylonica
3.947	-	glutathione	isoform GSTU12, at pH 6.5 and 25°C	Salix babylonica
4.781	-	1-chloro-2,4-dinitrobenzene	isoform GSTU4, at pH 6.5 and 25°C	Salix babylonica
6.664	-	1-chloro-2,4-dinitrobenzene	isoform GSTU11, at pH 6.5 and 25°C	Salix babylonica
7.562	-	1-chloro-2,4-dinitrobenzene	isoform GSTU9, at pH 6.5 and 25°C	Salix babylonica
9.797	-	1-chloro-2,4-dinitrobenzene	isoform GSTU14, at pH 6.5 and 25°C	Salix babylonica
11.472	-	1-chloro-2,4-dinitrobenzene	isoform GSTU13, at pH 6.5 and 25°C	Salix babylonica
13.277	-	glutathione	isoform GSTU1, at pH 6.5 and 25°C	Salix babylonica
14.073	-	glutathione	isoform GSTU5, at pH 6.5 and 25°C	Salix babylonica
20.296	-	glutathione	isoform GSTU4, at pH 6.5 and 25°C	Salix babylonica
22.285	-	glutathione	isoform GSTU11, at pH 6.5 and 25°C	Salix babylonica
25.022	-	glutathione	isoform GSTU14, at pH 6.5 and 25°C	Salix babylonica
26.053	-	glutathione	isoform GSTU10, at pH 6.5 and 25°C	Salix babylonica
28.733	-	1-chloro-2,4-dinitrobenzene	isoform GSTU3, at pH 6.5 and 25°C	Salix babylonica
30.102	-	1-chloro-2,4-dinitrobenzene	isoform GSTU1, at pH 6.5 and 25°C	Salix babylonica
36.365	-	glutathione	isoform GSTU9, at pH 6.5 and 25°C	Salix babylonica
43.529	-	glutathione	isoform GSTU13, at pH 6.5 and 25°C	Salix babylonica
49.862	-	1-chloro-2,4-dinitrobenzene	isoform GSTU6, at pH 6.5 and 25°C	Salix babylonica
116.572	-	glutathione	isoform GSTU6, at pH 6.5 and 25°C	Salix babylonica
119.3	-	glutathione	isoform GSTU3, at pH 6.5 and 25°C	Salix babylonica
7104	-	1-chloro-2,4-dinitrobenzene	isoform GSTU10, at pH 6.5 and 25°C	Salix babylonica