Literature summary for 2.5.1.18 extracted from

Di Matteo, A.; Federici, L.; Masulli, M.; Carletti, E.; Santorelli, D.; Cassidy, J.; Paradisi, F.; Di Ilio, C.; Allocati, N.
Structural characterization of the Xi class glutathione transferase from the haloalkaliphilic archaeon Natrialba magadii (2019), Front. Microbiol., 10, 9-9 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed as His6-tagged protein in Haloferax volcanii strain H1325	Natrialba magadii

Crystallization (Commentary)

Crystallization (Comment)	Organism
using 25% (w/v) polyethylene glycol 3350, 0.1 M Tris-HCl pH 8.5 and 0.2 M lithium sulfate	Natrialba magadii

Organism

Organism	UniProt	Comment	Textmining
Natrialba magadii	D3SS28	-	-
Natrialba magadii ATCC 43099	D3SS28	-	-

Purification (Commentary)

Purification (Comment)	Organism
His-Trap column chromatography and Superdex 200 gel filtration	Natrialba magadii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glutathione + 1-chloro-2,4-dinitrobenzene	-	Natrialba magadii	chloride + 2,4-dinitrophenyl-glutathione	-	?
glutathione + 1-chloro-2,4-dinitrobenzene	-	Natrialba magadii ATCC 43099	chloride + 2,4-dinitrophenyl-glutathione	-	?
glutathione + ethacrynic acid	-	Natrialba magadii	?	-	?
glutathione + ethacrynic acid	-	Natrialba magadii ATCC 43099	?	-	?
additional information	no thioltransferase activity with dehydroascorbic acid and 2-hydroxyethyldisulfide	Natrialba magadii	?	-	-
additional information	no thioltransferase activity with dehydroascorbic acid and 2-hydroxyethyldisulfide	Natrialba magadii ATCC 43099	?	-	-

Subunits

Subunits	Comment	Organism
?	x * about 45000, SDS-PAGE	Natrialba magadii

Synonyms

Synonyms	Comment	Organism
GHR	-	Natrialba magadii
GST	-	Natrialba magadii
Xi class glutathione transferase	-	Natrialba magadii

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Release 2023.1 (January 2023)