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Literature summary for 2.5.1.18 extracted from

  • Hirowatari, A.; Chen, Z.; Mita, K.; Yamamoto, K.
    Enzymatic characterization of two epsilon-class glutathione S-transferases of Spodoptera litura (2018), Arch. Insect Biochem. Physiol., 97, e21443 .
    View publication on PubMed
Search for more literature for 2.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) pLysS cells Spodoptera litura

Inhibitors

Inhibitors Comment Organism Structure
bendiocarb
-
 Spodoptera litura
chlorfenapyr
-
 Spodoptera litura
diazinon in the presence of 1 mM diazinon, isoform GST7 shows 23% of its original activity , whereas the activity of isoform GST20 is decreased to about 50% of its original activity Spodoptera litura
permethrin
-
 Spodoptera litura

Organism

Organism UniProt Comment Textmining
Spodoptera litura
-
 tobacco cutworm, strain Ishihara
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-affinity chromatography and Superdex S200 gel filtration Spodoptera litura

Source Tissue

Source Tissue Comment Organism Textmining
larva
-
 Spodoptera litura
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.19
-
 with 1-chloro-2,4-dinitrobenzene as substrate, isoform GST7, at pH 7.0, temperature not specified in the publication Spodoptera litura
0.56
-
 with 1,2-epoxy-3-(4-nitrophenoxy)-propane as substrate, isoform GST7, at pH 7.0, temperature not specified in the publication Spodoptera litura
0.63
-
 with 1,2-epoxy-3-(4-nitrophenoxy)-propane as substrate, isoform GST20, at pH 7.0, temperature not specified in the publication Spodoptera litura
0.83
-
 with 1-chloro-2,4-dinitrobenzene as substrate, isoform GST20, at pH 7.0, temperature not specified in the publication Spodoptera litura
1.3
-
 with ethacrynic acid as substrate, isoform GST7, at pH 7.0, temperature not specified in the publication Spodoptera litura
1.8
-
 with ethacrynic acid as substrate, isoform GST20, at pH 7.0, temperature not specified in the publication Spodoptera litura

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1,2-epoxy-3-(4-nitrophenoxy)-propane
-
 Spodoptera litura ?
-
 ?
glutathione + 1-chloro-2,4-dinitrobenzene
-
 Spodoptera litura S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
glutathione + ethacrynic acid highest activity Spodoptera litura ?
-
 ?
additional information no activity with 4-nitrobenzyl chloride, 4-nitrophenethyl bromide, 4-hydroxynonenal, 4-nitrophenyl acetate, and H2O2 Spodoptera litura ?
-
-

Subunits

Subunits Comment Organism
? x * 22281, isoform GST20, calculated from amino acid sequence Spodoptera litura
? x * 25942, isoform GST7, calculated from amino acid sequence Spodoptera litura

Synonyms

Synonyms Comment Organism
epsilon-class glutathione S-transferase
-
 Spodoptera litura
GST
-
 Spodoptera litura
GST20
-
 Spodoptera litura
GST7
-
 Spodoptera litura

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 60 isoform GST7 is stable at temperatures below 60°C for 30 min whereas isoform GST20 is stable at temperatures below 40°C for 30 min Spodoptera litura

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Spodoptera litura

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 11 after 24 h incubation, isoform GST7 retains more than 75% of its original activity in the range of pH 5.0-10.0. This pH range is narrower than that for isoform GST20 (which is 3.0-11.0) Spodoptera litura

pI Value

Organism Comment pI Value Maximum pI Value
Spodoptera litura isoform GST20, calculated from amino acid sequence
-
 6.5
Spodoptera litura isoform GST7, calculated from amino acid sequence
-
 8.7