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Literature summary for 2.5.1.18 extracted from

  • Mano, J.; Ishibashi, A.; Muneuchi, H.; Morita, C.; Sakai, H.; Biswas, M.S.; Koeduka, T.; Kitajima, S.
    Acrolein-detoxifying isozymes of glutathione transferase in plants (2017), Planta, 245, 255-264 .
    View publication on PubMed
Search for more literature for 2.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta2(DE3)pLysS cells Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0304
-
 acrolein at pH 6.0 and 25°C Arabidopsis thaliana
0.0512
-
 glutathione at pH 6.0 and 25°C Arabidopsis thaliana
0.0709
-
 glutathione at pH 6.0 and 25°C Spinacia oleracea
0.093
-
 acrolein at pH 6.0 and 25°C Spinacia oleracea
0.395
-
 4-hydroxy-(E)-2-nonenal at pH 6.0 and 25°C Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9ZRW8 ecotype Columbia-0
-
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
GSTrap 4B column chromatography, HiTrap Q column chromatography, and DEAE-Sepharose column chromatography Spinacia oleracea
GSTrap 4B column chromatography, HiTrap Q column chromatography, and DEAE-Sepharose column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-
leaf
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
 less than 0.01 micromol/min/mg with 0.1 mM 4-nitrobenzyl chloride as substrate, at pH 6.0 and 25°C Spinacia oleracea
0.01
-
 less than 0.01 micromol/min/mg with 0.1 mM benzyl isothiocyanate as substrate, at pH 6.0 and 25°C Spinacia oleracea
0.01
-
 less than 0.01 micromol/min/mg with 0.1 mM crotonaldehyde as substrate, at pH 6.0 and 25°C Spinacia oleracea
0.05
-
 purified protein with poly(His) tag, with 12-oxo-phytodienoic acid as substrate, at pH 6.0 and 25°C Arabidopsis thaliana
0.07
-
 with 0.1 mM 4-hydroxy-(E)-2-nonenal as substrate, at pH 6.0 and 25°C Spinacia oleracea
1.7
-
 with 0.1 mM acrolein as substrate, at pH 6.0 and 25°C Spinacia oleracea
3.83
-
 purified protein with poly(His) tag, with acrolein as substrate, at pH 6.0 and 25°C Arabidopsis thaliana
5.2
-
 with 0.1 mM 1-chloro-2,4-dinitrobenzene as substrate, at pH 6.0 and 25°C Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloro-2,4-dinitrobenzene + glutathione
-
 Spinacia oleracea S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
12-oxo-phytodienoic acid + glutathione
-
 Arabidopsis thaliana ?
-
 ?
4-hydroxy-(E)-2-nonenal + glutathione
-
 Spinacia oleracea ?
-
 ?
4-nitrobenzyl chloride + glutathione
-
 Spinacia oleracea S-(4-nitrobenzyl)glutathione + HCl
-
 ?
acrolein + glutathione
-
 Spinacia oleracea ?
-
 ?
acrolein + glutathione
-
 Arabidopsis thaliana ?
-
 ?
benzyl isothiocyanate + glutathione
-
 Spinacia oleracea ?
-
 ?

Subunits

Subunits Comment Organism
? x * 24000, SDS-PAGE Spinacia oleracea
? x * 29000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
GST
-
 Spinacia oleracea
GST Tau19
-
 Arabidopsis thaliana
GST-Acr isozyme Spinacia oleracea
GSTU19
-
 Arabidopsis thaliana
RX:glutathione R-transferase
-
 Spinacia oleracea
RX:glutathione R-transferase
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0709
-
 glutathione at pH 6.0 and 25°C Spinacia oleracea
0.262
-
 4-hydroxy-(E)-2-nonenal at pH 6.0 and 25°C Spinacia oleracea
1.15
-
 acrolein at pH 6.0 and 25°C Spinacia oleracea
1.78
-
 acrolein at pH 6.0 and 25°C Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Spinacia oleracea isoelectric focusing
-
 5.9

General Information

General Information Comment Organism
metabolism the enzyme keeps the acrolein level low in plant cells Spinacia oleracea
metabolism the enzyme keeps the acrolein level low in plant cells Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0709
-
 glutathione at pH 6.0 and 25°C Spinacia oleracea
0.664
-
 4-hydroxy-(E)-2-nonenal at pH 6.0 and 25°C Spinacia oleracea
12.3
-
 acrolein at pH 6.0 and 25°C Spinacia oleracea
59.3
-
 acrolein at pH 6.0 and 25°C Arabidopsis thaliana