Literature summary for 2.5.1.18 extracted from

Cui, J.; Li, L.G.; Jiang, P.; Liu, R.D.; Yang, X.; Liu, L.N.; Liu, P.; Zhang, S.B.; Wang, Z.Q.
Biochemical and functional characterization of the glutathione S-transferase from Trichinella spiralis (2015), Parasitol. Res., 114, 2007-2013.

Search for more literature for 2.5.1.18

Activating Compound

Activating Compound	Comment	Organism	Structure
dithiothreitol	153.6% activity at 10 mM	Trichinella spiralis

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	91.3% residual activity at 5 mM	Trichinella spiralis
H2O2	2.1% residual activity at 0.2% (v/v)	Trichinella spiralis
K+	93.7% residual activity at 5 mM	Trichinella spiralis
Mn2+	83.8% residual activity at 5 mM	Trichinella spiralis
Na+	81.7% residual activity at 5 mM	Trichinella spiralis
SDS	12.7% residual activity at 10 mM	Trichinella spiralis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.123	-	glutathione	at pH 6.5 and 25°C	Trichinella spiralis
0.457	-	1-chloro-2,4-dinitrobenzene	at pH 6.5 and 25°C	Trichinella spiralis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	not influenced by Mg2+	Trichinella spiralis
Ni2+	154.7% activity at 5 mM	Trichinella spiralis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24000	-	x * 24000, SDS-PAGE	Trichinella spiralis

Organism

Organism	UniProt	Comment	Textmining
Trichinella spiralis	-	isolate ISS534	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
larva	intestinal infective larva and muscle larva	Trichinella spiralis	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-chloro-2,4-dinitrobenzene + glutathione	-	Trichinella spiralis	S-(2,4-dinitrophenyl)glutathione + HCl	-	?

Subunits

Subunits	Comment	Organism
?	x * 24000, SDS-PAGE	Trichinella spiralis

Synonyms

Synonyms	Comment	Organism
glutathione S-transferase	-	Trichinella spiralis
GST	-	Trichinella spiralis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Trichinella spiralis

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	60	about 40% activity at 20°C, about 60% activity at 30°C, 100% activity at 40°C, about 60% activity at 50°C, about 40% activity at 60°C, about 20% activity at 70°C	Trichinella spiralis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
7.5	8	the enzyme is inactivated after exposure of 30 min in buffer of pH 7.5-8.0	Trichinella spiralis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.3	-	glutathione	at pH 6.5 and 25°C	Trichinella spiralis
18	-	1-chloro-2,4-dinitrobenzene	at pH 6.5 and 25°C	Trichinella spiralis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Trichinella spiralis

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	7	about 30% activity at pH 5.5-6.0, about 70% activity at pH 7.0	Trichinella spiralis

pI Value

Organism	Comment	pI Value Maximum	pI Value
Trichinella spiralis	calculated from amino acid sequence	-	6

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
9.3	-	glutathione	at pH 6.5 and 25°C	Trichinella spiralis
146.3	-	1-chloro-2,4-dinitrobenzene	at pH 6.5 and 25°C	Trichinella spiralis

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Release 2023.1 (January 2023)