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Literature summary for 2.5.1.18 extracted from

  • Stourman, N.V.; Branch, M.C.; Schaab, M.R.; Harp, J.M.; Ladner, J.E.; Armstrong, R.N.
    Structure and function of YghU, a nu-class glutathione transferase related to YfcG from Escherichia coli (2011), Biochemistry, 50, 1274-1281.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.18

Cloned(Commentary)

Cloned (Comment) Organism
gene yghU, expression in Escherichia coli strain BL21(DE3), expression of the selenomethionine YghU derivative in Escherichia coli strain B834 DE3 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant YghU, hanging-drop vapor diffusion method, YghU at 20 mg/ml in 30 mM NaH2PO4, pH 7.0, 1 mM DTT, and 20 mM GSH, is mixed with an equal volume of reservoir solution containing 0.1 M Bis-Tris, 0.2 M NaCl, pH 5.5, 25% w/v PEG 3350, 25°C, 1 week, X-ray diffraction structure determination and analysis at 1.5-2.5 A resolution Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
oxidized glutathione
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 gene yghU
-

Purification (Commentary)

Purification (Comment) Organism
recombinant YghU from strain BL21(DE3) by anion and cation exchange chromatography and dialysis Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloro-2,4-dinitrobenzene + glutathione
-
 Escherichia coli S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
additional information YghU from Escherichia coli binds two molecules of GSH in each active site. YghU exhibits little or no GSH transferase activity with most typical electrophilic substrates but does possess a modest catalytic activity toward several organic hydroperoxides. The enzyme also exhibits disulfide-bond reductase activity towards 2-hydroxyethyl disulfide Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
GSH transferase homologue
-
 Escherichia coli
nu-class glutathione transferase
-
 Escherichia coli
YghU
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7 assay at Escherichia coli

General Information

General Information Comment Organism
evolution structures and properties of YghU and YfcG indicate that they are members of the same distinct subfamily of GSH transferase homologues, proposed to be called the Nu-class GSH transferases. Cytoscape cluster analysis of the GSH transferase superfamily, overvew Escherichia coli