Cloned (Comment) | Organism |
---|---|
tau class GST gene OsGSTU17, DNA and amino acid sequence determination and analysis, overexpression of His6-tagged GSTU17 in Escherichia coli strain Bl21 | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.058 | - |
glutathione | pH 7.4, 37°C | Oryza sativa | |
0.324 | - |
7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole | pH 7.4, 37°C | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25180 | - |
x * 25180, GSTU17, sequence calculation | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q93WY5 | GSTU17; isozyme GSTU17 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bud | low expression of GSTU17 | Oryza sativa | - |
leaf | high expression of GSTU17 in mature, lower in young leaves, no expression in immature leaves | Oryza sativa | - |
root | low expression of GSTU17 | Oryza sativa | - |
stem | low expression of GSTU17 | Oryza sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.203 | - |
GSTU17, substrate 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Oryza sativa | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
glutathione + 4-nitrobenzo-2-oxa-1,3-diazole | - |
Oryza sativa | ? | - |
? | |
glutathione + 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole | - |
Oryza sativa | ? | - |
? | |
glutathione + cumene hydroperoxide | - |
Oryza sativa | ? | - |
? | |
additional information | no activity with 1,2-dichloro-4-nitrobenzene, 4-nitrophenyl acetate, and ethacrynic acid | Oryza sativa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25180, GSTU17, sequence calculation | Oryza sativa |
More | structure homology modeling | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
GSTU17 | - |
Oryza sativa |
tau class glutathione transferase | - |
Oryza sativa |
tau class GST | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
Oryza sativa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 65 | - |
Oryza sativa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.257 | - |
7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole | pH 7.4, 37°C | Oryza sativa | |
0.264 | - |
glutathione | pH 7.4, 37°C | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Oryza sativa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | activity range of GSTU17 | Oryza sativa |
General Information | Comment | Organism |
---|---|---|
metabolism | the classical phase II detoxification glutathione transferases are key metabolic enzymes that catalyze the conjugation of glutathione to various electrophilic compounds | Oryza sativa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole | pH 7.4, 37°C | Oryza sativa | |
0.076 | - |
glutathione | pH 7.4, 37°C | Oryza sativa |