Literature summary for 2.5.1.18 extracted from

Kapoli, P.; Axarli, I.A.; Platis, D.; Fragoulaki, M.; Paine, M.; Hemingway, J.; Vontas, J.; Labrou, N.E.
Engineering sensitive glutathione transferase for the detection of xenobiotics (2008), Biosens. Bioelectron., 24, 498-503.

Search for more literature for 2.5.1.18

Application

Application	Comment	Organism
analysis	immobilized GST mutant Gln53Ala is used to assemble a biosensor for malathion	Zea mays

Protein Variants

Protein Variants	Comment	Organism
Q53A	site-directed mutagenesis, the mutant of isozyme GST I exhibits 9.2fold higher inhibition potency for the insecticide malathion compared to the wild-type enzyme, mutant immobilization by crosslinking with glutaraldehyde for formation of a malathion biosensor, overview	Zea mays

General Stability

General Stability	Organism
the presence of GSH or glycerol at 20% v/v exhibits significant stabilizing effects on the purified enzyme mutant Q53A	Zea mays

Inhibitors

Inhibitors	Comment	Organism	Structure
malathion	competitive	Zea mays
additional information	bulky compounds with two aromatic rings, e.g. phenylurea-based insecticides, behave as strong inhibitors showing inhibition between 75% and 94%	Zea mays

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Zea mays	5829	-

Organism

Organism	UniProt	Comment	Textmining
Zea mays	P12653	isozyme GST I	-

Storage Stability

Storage Stability	Organism
4°C, purified mutant Q53A, 50 mM potassium phosphate buffer, pH 7.5, almost complete inactivation within 16 days, half-life of mutant inactivation is 12 days in the absence of stabilizers, or 18 days and 16 days in the presence of GSH and glycerol, respectively	Zea mays

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glutathione + 1-chloro-2,4-dinitrobenzene	-	Zea mays	S-(2,4-dinitrophenyl)glutathione + HCl	-	?

Synonyms

Synonyms	Comment	Organism
GST I	-	Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	37	assay at	Zea mays

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	assay at	Zea mays

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	inhibition kinetics of wild-type and mutant enzymes, overview	Zea mays
0.0297	-	malathion	pH 6.5, 37°C, versus 1-chloro-2,4-dinitrobenzene	Zea mays
0.0861	-	malathion	pH 6.5, 37°C, versus glutathione	Zea mays

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Release 2023.1 (January 2023)