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Literature summary for 2.5.1.18 extracted from

  • Aydemir, T.; Kavrayan, D.
    Purification and characterization of glutathione-S-transferase from chicken erythrocyte (2009), Artif. Cells Blood Substit. Immobil. Biotechnol., 37, 92-100.
    View publication on PubMed
Search for more literature for 2.5.1.18

Inhibitors

Inhibitors Comment Organism Structure
1,3-Bis-(2-chloroethyl)-1-nitrosourea competitive Gallus gallus
2-mercaptoethanol 50% inhibition at 4 mM Gallus gallus
Cd2+
-
 Gallus gallus
Cu2+
-
 Gallus gallus
DTNB
-
 Gallus gallus
DTT 50% inhibition at 10 mM Gallus gallus
EDTA
-
 Gallus gallus
FeCl3
-
 Gallus gallus
Hg2+ noncompetitive Gallus gallus
mercury acetate 50% inhibition at 28 nM Gallus gallus
Mn2+
-
 Gallus gallus
SDS
-
 Gallus gallus
Thiourea
-
 Gallus gallus
Triton X-100
-
 Gallus gallus
Urea
-
 Gallus gallus
Zn2+
-
 Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.71
-
 1,3-Bis-(2-chloroethyl)-1-nitrosourea pH 7.5, 28°C, GSH-S transferase rho Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 2 * 24000, GSH-S transferase rho, SDS-PAGE Gallus gallus
47500
-
 GSH-S transferase rho, gel filtration Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 GSH-S transferase rho
-

Purification (Commentary)

Purification (Comment) Organism
isozyme GSH-S transferase rho 340fold from erythrocytes by acetone precipitation, ethanol-chloroform treatment, two different steps of anion exchange chromatography, and gel filtration Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.39
-
 purified native GSH-S transferase rho Gallus gallus

Storage Stability

Storage Stability Organism
-15°C, purified enzyme, loss of 10% activity after 5 days Gallus gallus
28°C, purified enzyme, loss of activity after 5 days, crude extract shows loss of activity after 2 days Gallus gallus
4°C, purified enzyme, loss of activity after 3 days Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-bis-(2-chloroethyl)-1-nitrosourea + glutathione
-
 Gallus gallus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 24000, GSH-S transferase rho, SDS-PAGE Gallus gallus

Synonyms

Synonyms Comment Organism
glutathione-S-transferase
-
 Gallus gallus
GSH-S transferase rho
-
 Gallus gallus
More the enzyme belongs to the glutathione-S-transferase family of multifunctional enzymes Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
-
 Gallus gallus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10
-
 60 min, purified GSH-S transferase rho, no loss of activity Gallus gallus
20
-
 60 min, purified GSH-S transferase rho, loss of about 5% activity Gallus gallus
30
-
 60 min, purified GSH-S transferase rho, loss of 40% activity Gallus gallus
40
-
 60 min, purified GSH-S transferase rho, loss of over 95% activity Gallus gallus
50
-
 5 min, purified GSH-S transferase rho, complete loss of activity Gallus gallus
60
-
 2 min, purified GSH-S transferase rho, complete loss of activity Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Gallus gallus

pH Range

pH Minimum pH Maximum Comment Organism
3 10 activity pH profile of GSH-S transferase rho, overview Gallus gallus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 60 min, purified GSH-S transferase rho, loss of 70% activity Gallus gallus
5
-
 60 min, purified GSH-S transferase rho, loss of 65% activity Gallus gallus
6
-
 60 min, purified GSH-S transferase rho, loss of 55% activity Gallus gallus
7
-
 60 min, purified GSH-S transferase rho, loss of 25% activity Gallus gallus
7.5
-
 60 min, purified GSH-S transferase rho, no loss of activity Gallus gallus
8
-
 60 min, purified GSH-S transferase rho, loss of 25% activity Gallus gallus
9
-
 60 min, purified GSH-S transferase rho, loss of 40% activity Gallus gallus

General Information

General Information Comment Organism
physiological function the glutathione-S-transferases are a family of multifunctional enzymes involved in the detoxification of electrophilic xenobiotics primarily through conjugation to reduced glutathione Gallus gallus