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Literature summary for 2.5.1.18 extracted from
- Biochemical analysis of a recombinant glutathione transferase from the cestode Echinococcus granulosus (2010), Acta Trop., 114, 31-36.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| EgGST, expression of the cytosolic isozyme in Escherichia coli strain BL21(DE3) | Echinococcus granulosus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| albendazole | - |
Echinococcus granulosus |  |
| alizarin | - |
Echinococcus granulosus | |
| bilirubin | - |
Echinococcus granulosus | |
| bithionol | - |
Echinococcus granulosus | |
| bromosulfophthalein | - |
Echinococcus granulosus | |
| cibacron | - |
Echinococcus granulosus | |
| Cibacron blue | - |
Echinococcus granulosus | |
| closantel | - |
Echinococcus granulosus | |
| ellagic acid | - |
Echinococcus granulosus | |
| febendazole | - |
Echinococcus granulosus | |
| Hexachlorophene | - |
Echinococcus granulosus | |
| mebendazole | - |
Echinococcus granulosus | |
| additional information | no inhibition of the cytosolic isozyme by chenodeoxycholic acid and 4-nitropyridine-N-oxide | Echinococcus granulosus | |
| Rafoxanide | - |
Echinococcus granulosus | |
| triphenylthin chloride | - |
Echinococcus granulosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten steady-state kinetic studies, recombinant cytosolic isozyme, overview | Echinococcus granulosus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | cytosolic isozyme | Echinococcus granulosus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25600 | - |
2 * 25600, recombinant cytosolic isozyme, SDS-PAGE | Echinococcus granulosus |
| 50000 | - |
recombinant cytosolic isozyme, gel filtration | Echinococcus granulosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Echinococcus granulosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant cytosolic isozyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Echinococcus granulosus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.5 | - |
purified recombinant cytosolic isozyme, substrate 1-chloro-2.4-dinitrobenzene | Echinococcus granulosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + 1-chloro-2,4-dinitrobenzene | - |
Echinococcus granulosus | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
| glutathione + cumene hydroperoxide | - |
Echinococcus granulosus | ? | - |
? | |
| glutathione + ethacrynic acid | - |
Echinococcus granulosus | ? | - |
? | |
| glutathione + trans,trans-2,4-decadienal | - |
Echinococcus granulosus | ? | - |
? | |
| glutathione + trans-2-nonenal | - |
Echinococcus granulosus | ? | - |
? | |
| additional information | the recombinant cytosolic GST displays glutathione peroxidase activity towards cumene hydroperoxide, and conjugates reactive carbonyls, trans-2-nonenal and trans,trans-2,4-decadienal, indicating that it may intercept damaging products of lipid peroxidation | Echinococcus granulosus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 25600, recombinant cytosolic isozyme, SDS-PAGE | Echinococcus granulosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GST | - |
Echinococcus granulosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Echinococcus granulosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 55 | - |
Echinococcus granulosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Echinococcus granulosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | - |
Echinococcus granulosus |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00012 | - |
pH 6.5, 25°C, recombinant cytosolic isozyme | Echinococcus granulosus | Cibacron blue | |
| 0.0023 | - |
pH 6.5, 25°C, recombinant cytosolic isozyme | Echinococcus granulosus | triphenylthin chloride | |
| 0.0041 | - |
pH 6.5, 25°C, recombinant cytosolic isozyme | Echinococcus granulosus | Hexachlorophene | |
| 0.0073 | - |
pH 6.5, 25°C, recombinant cytosolic isozyme | Echinococcus granulosus | ellagic acid | |
| 0.039 | - |
pH 6.5, 25°C, recombinant cytosolic isozyme | Echinococcus granulosus | Rafoxanide | |
| 0.05 | - |
above, pH 6.5, 25°C, recombinant cytosolic isozyme | Echinococcus granulosus | closantel | |
| 0.05 | - |
above, pH 6.5, 25°C, recombinant cytosolic isozyme | Echinococcus granulosus | febendazole | |
| 0.065 | - |
pH 6.5, 25°C, recombinant cytosolic isozyme | Echinococcus granulosus | alizarin | |
| 0.126 | - |
pH 6.5, 25°C, recombinant cytosolic isozyme | Echinococcus granulosus | bithionol | |
| 0.16 | - |
pH 6.5, 25°C, recombinant cytosolic isozyme | Echinococcus granulosus | albendazole | |
| 0.165 | - |
pH 6.5, 25°C, recombinant cytosolic isozyme | Echinococcus granulosus | bilirubin | |
| 0.188 | - |
pH 6.5, 25°C, recombinant cytosolic isozyme | Echinococcus granulosus | bromosulfophthalein | |
| 0.5 | - |
above, pH 6.5, 25°C, recombinant cytosolic isozyme | Echinococcus granulosus | mebendazole | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | glutathione transferases are a major detoxification system in helminths | Echinococcus granulosus |
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