Literature summary for 2.5.1.18 extracted from

Remmerie, B.; Vandenbroucke, K.; De Smet, L.; Carpentier, W.; De Vos, D.; Stout, J.; Van Beeumen, J.; Savvides, S.N.
Expression, purification, crystallization and structure determination of two glutathione S-transferase-like proteins from Shewanella oneidensis (2008), Acta Crystallogr. Sect. F, 64, 548-553.

Cloned(Commentary)

Cloned (Comment)	Organism
isozymes SoGST3 and SoGST6, expression in Escherichia coli strain BL21 (DE3)	Shewanella oneidensis

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant isozymes SoGST3 and SoGST6, hanging drop vapour diffusion method, 7 mg/ml protein, isozyme SoGST3 gives two crystal forms: SoGST3-A crystals grow from 22% w/v PEG 4000, 0.1 M CHES, pH 9, 0.25 M calcium chloride, 4 mM GSH, and SoGST3-B crystals grow from 22% w/v PEG 4000, 0.1 M CHES, pH 9, 0.25 M calcium chloride, 2 mM GSH and 0.5 mM DTT, isozyme SoGST6 crystals grow within 3 d at 21°C, from 15% w/v PEG 3350, 50 mM MES, pH 6.3, 0.2 M sodium chloride and 0-0.3 mM GSH, X-ray diffraction structure determination and analysis at 2.6-4.0 A resolution	Shewanella oneidensis

Crystallization (Comment)

Organism

purified recombinant isozymes SoGST3 and SoGST6, hanging drop vapour diffusion method, 7 mg/ml protein, isozyme SoGST3 gives two crystal forms: SoGST3-A crystals grow from 22% w/v PEG 4000, 0.1 M CHES, pH 9, 0.25 M calcium chloride, 4 mM GSH, and SoGST3-B crystals grow from 22% w/v PEG 4000, 0.1 M CHES, pH 9, 0.25 M calcium chloride, 2 mM GSH and 0.5 mM DTT, isozyme SoGST6 crystals grow within 3 d at 21°C, from 15% w/v PEG 3350, 50 mM MES, pH 6.3, 0.2 M sodium chloride and 0-0.3 mM GSH, X-ray diffraction structure determination and analysis at 2.6-4.0 A resolution

Shewanella oneidensis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Shewanella oneidensis	5829	-

Organism

Organism	UniProt	Comment	Textmining
Shewanella oneidensis	-	six glutathione S-transferase-like genes, sogst1-sogst6	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant isozymes SoGST3 and SoGST6 from Escherichia coli strain BL21 (DE3) by anion exchange chromatography, ammonium sulfate fractionation, cation exchange chromatography, and again two steps of anion exchange chromatography on different resins	Shewanella oneidensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	structure-function study of glutathione transferase-like proteins, overview	Shewanella oneidensis	?	-	?

Subunits

Subunits	Comment	Organism
More	structure-function study of glutathione transferase-like proteins, Structure-based multiple sequence alignments, overview	Shewanella oneidensis

Synonyms

Synonyms	Comment	Organism
glutathione S-transferase	-	Shewanella oneidensis
glutathione transferase-like protein	-	Shewanella oneidensis
GST	-	Shewanella oneidensis