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Literature summary for 2.5.1.18 extracted from

  • Del Buono, D.; Scarponi, L.; Espen, L.
    Glutathione S-transferases in Festuca arundinacea: identification, characterization and inducibility by safener benoxacor (2007), Phytochemistry, 68, 2614-2624.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
benoxacor induces 200% content increase of isozyme GST I in treated shoots Lolium arundinaceum
benoxacor induces 30% content increase of isozyme GST II in treated shoots Lolium arundinaceum
additional information not activated by benoxacor Lolium arundinaceum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
1 * 27500 + 1 * 26000, gel filtration Lolium arundinaceum
27200
-
1 * 28000 + 1 * 27200, gel filtration Lolium arundinaceum
27500
-
1 * 27500 + 1 * 26000, gel filtration Lolium arundinaceum
27500
-
1 * 30000 + 1 * 27500, gel filtration Lolium arundinaceum
28000
-
1 * 28000 + 1 * 27200, gel filtration Lolium arundinaceum
28000
-
1 * 29000 + 1 * 28000, gel filtration Lolium arundinaceum
29000
-
1 * 29000 + 1 * 28000, gel filtration Lolium arundinaceum
30000
-
1 * 30000 + 1 * 27500, gel filtration Lolium arundinaceum

Organism

Organism UniProt Comment Textmining
Lolium arundinaceum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Q Sepharose column chromatography, GSTrap column chromatography, and High Q column chromatography Lolium arundinaceum

Source Tissue

Source Tissue Comment Organism Textmining
shoot
-
Lolium arundinaceum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.165
-
after 162fold purification Lolium arundinaceum
0.51
-
after 162fold purification Lolium arundinaceum
34.55
-
after 162fold purification Lolium arundinaceum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
-
Lolium arundinaceum S-(2,4-dinitrophenyl)glutathione + HCl
-
?

Subunits

Subunits Comment Organism
heterodimer 1 * 27500 + 1 * 26000, gel filtration Lolium arundinaceum
heterodimer 1 * 28000 + 1 * 27200, gel filtration Lolium arundinaceum
heterodimer 1 * 29000 + 1 * 28000, gel filtration Lolium arundinaceum
heterodimer 1 * 30000 + 1 * 27500, gel filtration Lolium arundinaceum

Synonyms

Synonyms Comment Organism
glutathione S-transferase
-
Lolium arundinaceum
GST I isozyme Lolium arundinaceum
GST II isozyme Lolium arundinaceum
GST III isozyme Lolium arundinaceum
GST IV isozyme Lolium arundinaceum