Any feedback?
Literature summary for 2.5.1.18 extracted from
- Glutathione mediated regulation of oligomeric structure and functional activity of Plasmodium falciparum glutathione S-transferase (2007), BMC Struct. Biol., 7, 67.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli M15 cells | Plasmodium falciparum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
4 * 26000, gel filtration, inactive enzyme form under native conditions | Plasmodium falciparum |
| 104000 | - |
gel filtration | Plasmodium falciparum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose chromatography | Plasmodium falciparum |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | active enzyme form, in the presence of 2 mM glutathione | Plasmodium falciparum |
| tetramer | 4 * 26000, gel filtration, inactive enzyme form under native conditions | Plasmodium falciparum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione S-transferase | - |
Plasmodium falciparum |
| GST | - |
Plasmodium falciparum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | for the GST dimer, the secondary structure is stable between pH 5.0 and 8.0, decrease in pH below 5.0 or increase in pH above 8.0 results in denaturation of the enzyme as indicated by a significant loss in secondary structure, at pH 4.0 or below and pH 10.0 almost complete loss of secondary structure is observed, for the GST tetramer a sigmoidal dependence of the loss of secondary structure on the pH value is observed, between pH 10.0 and 6.0 no alteration is determined, decrease in pH below 6.0 results in loss of secondary structure, even at a pH as low as 3.0 only a partial loss of secondary structure of about 50% is observed | Plasmodium falciparum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
