Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysSL cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
E80Q | 7% residual activity compared to the wild type enzyme | Rattus norvegicus |
H75Q | 34% residual activity compared to the wild type enzyme | Rattus norvegicus |
N77T | inactive | Rattus norvegicus |
R113K | 5% residual activity compared to the wild type enzyme | Rattus norvegicus |
R63A | 30% increased activity compared to the wild type enzyme | Rattus norvegicus |
R72A | 42% residual activity compared to the wild type enzyme | Rattus norvegicus |
R73Q | 57% residual activity compared to the wild type enzyme | Rattus norvegicus |
S30A | 400% increased activity compared to the wild type enzyme | Rattus norvegicus |
T64V | 280% increased activity compared to the wild type enzyme | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Rattus norvegicus | 16020 | - |
microsome | - |
Rattus norvegicus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P08011 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.6 | - |
wild type enzyme, using 1-chloro-2,4-dinitrobenzene as a substrate | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Rattus norvegicus | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | - |
Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
MGST1 | - |
Rattus norvegicus |
microsomal glutathione transferase-1 | membrane-bound enzyme with both glutathione transferase and hydroperoxidase activities | Rattus norvegicus |