Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.18 extracted from

  • Trute, M.; Gallis, B.; Doneanu, C.; Shaffer, S.; Goodlett, D.; Gallagher, E.
    Characterization of hepatic glutathione S-transferases in coho salmon (Oncorhynchus kisutch) (2007), Aquat. Toxicol., 81, 126-136.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.18

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Oncorhynchus kisutch 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Oncorhynchus kisutch a phase II detoxification enzyme which protect against chemical injury, especially in the context of detoxifying waterborne pollutants ?
-
 ?

Organism

Organism UniProt Comment Textmining
Oncorhynchus kisutch L7QHV5
-
-
Oncorhynchus kisutch L7QHV5 coho salmon, several isozymes, e.g. pi and rho-class GSTs, as well as alpha and mu class isozymes
-
Oncorhynchus kisutch L7QI27
-
-

Purification (Commentary)

Purification (Comment) Organism
native cytosolic liver GST 97fold by glutathione affinity chromatography Oncorhynchus kisutch

Source Tissue

Source Tissue Comment Organism Textmining
liver several isozymes Oncorhynchus kisutch
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
31.2
-
 purified liver GST, substrate 1-chloro-2,4-dinitrobenzene Oncorhynchus kisutch

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene preferred substrate Oncorhynchus kisutch chloride + 2,4-dinitrophenyl-glutathione
-
 ?
glutathione + ethacrynic acid low activity Oncorhynchus kisutch ?
-
 ?
glutathione + nitrobutyl chloride
-
 Oncorhynchus kisutch chloride + nitrobutyl-glutathione
-
 ?
additional information a phase II detoxification enzyme which protect against chemical injury, especially in the context of detoxifying waterborne pollutants Oncorhynchus kisutch ?
-
 ?
additional information substrate specificity, overview, poor activity with cumene hydroperoxide, 4-hydroxynonenal, methyl parathion or atrazine Oncorhynchus kisutch ?
-
 ?

Subunits

Subunits Comment Organism
? x * 24000-26000, liver enzyme, SDS-PAGE Oncorhynchus kisutch
More isozyme identification by Fourier transform-ion cyclotron resonance mass spectrometer analysis, overview Oncorhynchus kisutch

Synonyms

Synonyms Comment Organism
glutathione S-transferase
-
 Oncorhynchus kisutch
GST
-
 Oncorhynchus kisutch
More the enzyme belongs to the family of glutathione S-transferases Oncorhynchus kisutch

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
 assay at Oncorhynchus kisutch

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 8.5 assay at, substrate-dependent Oncorhynchus kisutch