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Literature summary for 2.5.1.18 extracted from

  • Willmore, W.G.; Storey, K.B.
    Purification and properties of the glutathione S-transferases from the anoxia-tolerant turtle,Trachemys scripta elegans (2005), FEBS J., 272, 3602-3614.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cibacron blue IC50: 0.048 mM for homodimeric enzyme, IC50: 0.0084 mM for heterodimeric enzyme Trachemys scripta elegans
GSSG IC50: 2.0 mM for homodimeric enzyme, IC50: 2.6 mM for heterodimeric enzyme Trachemys scripta elegans
iodoacetamide IC50: 40 mM for homodimeric enzyme, 8.7 mM for heterodimeric enzyme Trachemys scripta elegans
KCl IC50: 0.33 M for homodimeric enzyme, IC50: 0.18 M for heterodimeric enzyme Trachemys scripta elegans
NaCl IC50: 0.332 M for homodimeric enzyme, IC50: 0.17 M for heterodimeric enzyme Trachemys scripta elegans
NH4Cl IC50: 0.2 M for homodimeric enzyme, IC50: 0.12 M for heterodimeric enzyme Trachemys scripta elegans
Rose bengal IC50: 0.00031 mM for homodimeric enzyme, IC50: 0.00047 mM for heterodimeric enzyme Trachemys scripta elegans
S-hexylglutathione IC50: 0.00031 mM for homodimeric enzyme, IC50: 0.00039 mM for heterodimeric enzyme Trachemys scripta elegans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
GSH homodimeric enzyme Trachemys scripta elegans
0.6
-
GSH heterodimeric enzyme Trachemys scripta elegans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32600
-
1 * 32600 + 1 * 36800, enzyme occurs as homodimeric form and as heterodimeric form, SDS-PAGE Trachemys scripta elegans
34000
-
2 * 34000, enzyme occurs as homodimeric form and as heterodimeric form, SDS-PAGE Trachemys scripta elegans
36800
-
1 * 32600 + 1 * 36800, enzyme occurs as homodimeric form and as heterodimeric form, SDS-PAGE Trachemys scripta elegans

Organism

Organism UniProt Comment Textmining
Trachemys scripta elegans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Trachemys scripta elegans

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Trachemys scripta elegans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-chloro-2,4-dinitrobenzene + GSH
-
Trachemys scripta elegans S-(2,4-dinitrophenyl)glutathione + HCl
-
?
cumene hydroperoxide + GSH
-
Trachemys scripta elegans ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 32600 + 1 * 36800, enzyme occurs as homodimeric form and as heterodimeric form, SDS-PAGE Trachemys scripta elegans
dimer 2 * 34000, enzyme occurs as homodimeric form and as heterodimeric form, SDS-PAGE Trachemys scripta elegans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Trachemys scripta elegans

pH Range

pH Minimum pH Maximum Comment Organism
6.3 7.4 pH 6.3: about 70% of maximal activity, pH 7.4: about 40% of maximal activity Trachemys scripta elegans

pI Value

Organism Comment pI Value Maximum pI Value
Trachemys scripta elegans
-
-
additional information

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00039
-
IC50: 0.00031 mM for homodimeric enzyme, IC50: 0.00039 mM for heterodimeric enzyme Trachemys scripta elegans S-hexylglutathione
0.00047
-
IC50: 0.00031 mM for homodimeric enzyme, IC50: 0.00047 mM for heterodimeric enzyme Trachemys scripta elegans Rose bengal
0.0084
-
IC50: 0.048 mM for homodimeric enzyme, IC50: 0.0084 mM for heterodimeric enzyme Trachemys scripta elegans Cibacron blue
2.6
-
IC50: 2.0 mM for homodimeric enzyme, IC50: 2.6 mM for heterodimeric enzyme Trachemys scripta elegans GSSG
40
-
IC50: 40 mM for homodimeric enzyme, 8.7 mM for heterodimeric enzyme Trachemys scripta elegans iodoacetamide
120
-
IC50: 0.12 M for heterodimeric enzyme Trachemys scripta elegans NH4Cl
170
-
IC50: 0.17 M for heterodimeric enzyme Trachemys scripta elegans NaCl
180
-
IC50: 0.18 M for heterodimeric enzyme Trachemys scripta elegans KCl
200
-
IC50: 0.2 M for homodimeric enzyme Trachemys scripta elegans NH4Cl
330
-
IC50: 0.33 M for homodimeric enzyme Trachemys scripta elegans KCl
332
-
IC50: 0.332 M for homodimeric enzyme Trachemys scripta elegans NaCl