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Literature summary for 2.5.1.18 extracted from
- Molecular characterization of a glutathione transferase from Pinus tabulaeformis (Pinaceae) (2005), Biochimie, 87, 445-455.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pinus tabuliformis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S13A | mutant enzyme shows no significant GST activity with 1-chloro-2,3-dinitrobenzene or with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole | Pinus tabuliformis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.47 | - |
1-chloro-2,3-dinitrobenzene | - |
Pinus tabuliformis |  |
| 0.83 | - |
GSH | reaction with 1-chloro-2,3-dinitrobenzene | Pinus tabuliformis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31800 | - |
2 * 31800, SDS-PAGE | Pinus tabuliformis |
| 60000 | - |
gel filtration | Pinus tabuliformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pinus tabuliformis | Q6DNI8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Pinus tabuliformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 169.1 | - |
- |
Pinus tabuliformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-chloro-2,3-dinitrobenzene + GSH | - |
Pinus tabuliformis | ? | - |
? | |
| 4-nitrophenyl acetate + GSH | 0.2% of the activity with 1-chloro-2,3-dinitrobenzene | Pinus tabuliformis | ? | - |
? | |
| 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole + GSH | 23.4% of the activity with 1-chloro-2,3-dinitrobenzene | Pinus tabuliformis | ? | - |
? | |
| ethacrynic acid + GSH | 8% of the activity with 1-chloro-2,3-dinitrobenzene | Pinus tabuliformis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 31800, SDS-PAGE | Pinus tabuliformis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PtGSTU1 | - |
Pinus tabuliformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 35 | - |
Pinus tabuliformis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 50 | more than 60% of maximal activity at 15°C and at 45°C, 50°C: 22% of maximal activity | Pinus tabuliformis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6 | - |
1-chloro-2,3-dinitrobenzene | - |
Pinus tabuliformis | |
| 6.7 | - |
GSH | reaction with 1-chloro-2,3-dinitrobenzene | Pinus tabuliformis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Pinus tabuliformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | pH 7.5: 56% of maximal activity, pH 9.5: 49% of maximal activity, enzyme activity at pH 5.5 and pH 10.5 is nearly abolished | Pinus tabuliformis |
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