Cloned (Comment) | Organism |
---|---|
production of recombinant selenoproteins with internal selenocysteine residues in Escherichia col, introduction of a variant bacterial-type selenocysteine insertion sequence element which afforded substitution with selenocysteine for the catalytic Tyr residue in the active site of the enzyme | Schistosoma japonicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
GSH | wild-type enzyme | Schistosoma japonicum | |
0.57 | - |
GSH | selenoenzyme | Schistosoma japonicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
selenium | production of recombinant selenoproteins with internal selenocysteine residues in Escherichia coli. Introduction of a selenocysteine residue into the catalytic site of glutathione S-transferase is not sufficient to induce glutathione peroxidase activity in spite of a maintained glutathione-binding capacity | Schistosoma japonicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schistosoma japonicum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-chloro-2,4-dinitrobenzene + GSH | - |
Schistosoma japonicum | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? |
Synonyms | Comment | Organism |
---|---|---|
sjGST | - |
Schistosoma japonicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
GSH | selenoenzyme | Schistosoma japonicum | |
16.6 | - |
GSH | wild-type enzyme | Schistosoma japonicum |