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Literature summary for 2.5.1.18 extracted from

  • Mozer, T.J.; Tiemeier, D.C.; Jaworski, E.G.
    Purification and characterization of corn glutathione S-transferase (1983), Biochemistry, 22, 1068-1072.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
alachlor i.e. 2-chloro-N-(2,6-diethylphenyl)-N-(methoxymethyl)acetamide Zea mays
2.8
-
1-chloro-2,4-dinitrobenzene GST II Zea mays
3
-
1-chloro-2,4-dinitrobenzene GST I Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
K+ no enzyme activity with potassium phosphate concentrations below 20 mM, optimal concentration 50 mM Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
2 * 29000, enzyme GST II, SDS-PAGE Zea mays
29000
-
2 * 29000, enzyme GST I, SDS-PAGE Zea mays
50000
-
gel filtration Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
2 forms: GST I, GST II
-

Purification (Commentary)

Purification (Comment) Organism
2 forms: GST I, GST II Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
leaf etiolated Zea mays
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21
-
GST II Zea mays
66
-
GST I Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + alachlor i.e. 2-chloro-N-(2,6-diethylphenyl)-N-(methoxymethyl)acetamide Zea mays ?
-
?
RX + glutathione RX: R: aliphatic, aromatic or heterocyclic, X: sulfate, nitrite or halide, enzyme also catalyzes: the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrite, certain isomerization reactions and disulfide interchange Zea mays HX + R-S-glutathione
-
?

Subunits

Subunits Comment Organism
dimer 2 * 29000, enzyme GST II, SDS-PAGE Zea mays
dimer 2 * 29000, enzyme GST I, SDS-PAGE Zea mays