Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as an N-terminal His-tagged fusion protein | Homo sapiens |
expressed in Escherichia coli as an N-terminal His-tagged fusion protein | Limosilactobacillus reuteri |
Protein Variants | Comment | Organism |
---|---|---|
F112A | mutation of the conserved Phe-112 in the active site of PduO is critical for the displacement of cob(II)alamin and leads to an inactive enzyme | Limosilactobacillus reuteri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cob(I)alamin | Limosilactobacillus reuteri | - |
triphosphate + adenosylcob(III)alamin | adenosyltransferase enzymes lower the thermodynamic barrier of the Co2+ to Co+ reduction needed for the formation of the unique organometalic Co-C bond of adenosylcobalamin | ? | |
ATP + cob(I)alamin | Homo sapiens | - |
triphosphate + adenosylcob(III)alamin | adenosyltransferase enzymes lower the thermodynamic barrier of the Co2+ toCo+ reduction needed for the formation of the unique organometalic Co-C bond of adenosylcobalamin | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Limosilactobacillus reuteri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a HisTrap FF column | Homo sapiens |
using a HisTrap FF column | Limosilactobacillus reuteri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.023 | - |
reductant: PduS protein, specific activity of LrPduO using different electron sources for the adenosylation of cob(II)alamin | Limosilactobacillus reuteri |
0.026 | - |
reductant: PduS protein, specific activity of hATR using different electron sources for the adenosylation of cob(II)alamin | Homo sapiens |
0.045 | - |
reductant: NADPH-dependent ferredoxin protein reductase, specific activity of LrPduO using different electron sources for the adenosylation of cob(II)alamin | Limosilactobacillus reuteri |
0.072 | - |
reductant: NADPH-dependent ferredoxin protein reductase, specific activity of hATR using different electron sources for the adenosylation of cob(II)alamin | Homo sapiens |
0.14 | - |
reductant: dihydroflavin, specific activity of LrPduO using different electron sources for the adenosylation of cob(II)alamin | Limosilactobacillus reuteri |
0.25 | - |
reductant: dihydroflavin, specific activity of hATR using different electron sources for the adenosylation of cob(II)alamin | Homo sapiens |
0.25 | - |
reductant: Ti(III)citrate, specific activity of LrPduO using different electron sources for the adenosylation of cob(II)alamin | Limosilactobacillus reuteri |
0.49 | - |
reductant: Ti(III)citrate, specific activity of hATR using different electron sources for the adenosylation of cob(II)alamin | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cob(I)alamin | - |
Limosilactobacillus reuteri | triphosphate + adenosylcob(III)alamin | - |
? | |
ATP + cob(I)alamin | - |
Limosilactobacillus reuteri | triphosphate + adenosylcob(III)alamin | adenosyltransferase enzymes lower the thermodynamic barrier of the Co2+ to Co+ reduction needed for the formation of the unique organometalic Co-C bond of adenosylcobalamin | ? | |
ATP + cob(I)alamin | - |
Homo sapiens | triphosphate + adenosylcob(III)alamin | adenosyltransferase enzymes lower the thermodynamic barrier of the Co2+ toCo+ reduction needed for the formation of the unique organometalic Co-C bond of adenosylcobalamin | ? |
Synonyms | Comment | Organism |
---|---|---|
hATR | - |
Homo sapiens |
LrPduO | - |
Limosilactobacillus reuteri |
PduO-type corrinoid adenosyltransferase | - |
Homo sapiens |
PduO-type corrinoid adenosyltransferase | - |
Limosilactobacillus reuteri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Limosilactobacillus reuteri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
8 | - |
assay at | Limosilactobacillus reuteri |