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Literature summary for 2.5.1.17 extracted from

  • St.Maurice, M.; Mera, P.E.; Taranto, M.P.; Sesma, F.; Escalante-Semerena, J.C.; Rayment, I.
    Structural characterization of the active site of the PduO-type ATP:Co(I)rrinoid adenosyltransferase from Lactobacillus reuteri (2007), J. Biol. Chem., 282, 2596-2605.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene pduO, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Limosilactobacillus reuteri

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged enzyme in complex with its substrates, hanging drop vapour diffusion method, 20°C, 0.004 ml of 20 mg/ml protein in 10 mM Tris-HCl, pH 8.0, is mixed with 0.004 ml od precipitant solution containing 0.1 M HEPES, pH 8.5, 1.1 M ammonium sulfate, 2 mM ATP, 55 mM MgCl2, 165 mM NaCl, and 15 mM cob(I)alamin, 7 days, X-ray diffraction structure determination and analysis at 1.68 A resolution Limosilactobacillus reuteri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00013
-
cob(I)alamin
-
Limosilactobacillus reuteri
0.0022
-
ATP
-
Limosilactobacillus reuteri

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Limosilactobacillus reuteri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cob(I)alamin + ATP Limosilactobacillus reuteri adenosylation of the corrinoid ring of cob(I)alamin generates coenzyme B12, i.e. adenosylcobalamin or AdoCbl, an essential cofactor used by enzymes that catalyze intramolecular rearrangements, deaminations, dehydrations, reductions, and reductive dehalogenations adenosylcobalamin + triphosphate
-
?
cob(I)alamin + ATP Limosilactobacillus reuteri CRL1098 adenosylation of the corrinoid ring of cob(I)alamin generates coenzyme B12, i.e. adenosylcobalamin or AdoCbl, an essential cofactor used by enzymes that catalyze intramolecular rearrangements, deaminations, dehydrations, reductions, and reductive dehalogenations adenosylcobalamin + triphosphate
-
?

Organism

Organism UniProt Comment Textmining
Limosilactobacillus reuteri
-
gene pduO
-
Limosilactobacillus reuteri CRL1098
-
gene pduO
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) Limosilactobacillus reuteri

Reaction

Reaction Comment Organism Reaction ID
2 ATP + 2 cob(II)alamin + a reduced flavoprotein = 2 triphosphate + 2 adenosylcob(III)alamin + an oxidized flavoprotein active site and substrate binding sites and structures, molecular architecture Limosilactobacillus reuteri
2 ATP + 2 cob(II)yrinic acid a,c-diamide + a reduced flavoprotein = 2 triphosphate + 2 adenosylcob(III)yrinic acid a,c-diamide + an oxidized flavoprotein active site and substrate binding sites and structures, molecular architecture Limosilactobacillus reuteri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cob(I)alamin + ATP adenosylation of the corrinoid ring of cob(I)alamin generates coenzyme B12, i.e. adenosylcobalamin or AdoCbl, an essential cofactor used by enzymes that catalyze intramolecular rearrangements, deaminations, dehydrations, reductions, and reductive dehalogenations Limosilactobacillus reuteri adenosylcobalamin + triphosphate
-
?
cob(I)alamin + ATP adenosylation of the corrinoid ring of cob(I)alamin, active site structure, ATP binding motif at the protein N terminus Limosilactobacillus reuteri adenosylcobalamin + triphosphate
-
?
cob(I)alamin + ATP adenosylation of the corrinoid ring of cob(I)alamin generates coenzyme B12, i.e. adenosylcobalamin or AdoCbl, an essential cofactor used by enzymes that catalyze intramolecular rearrangements, deaminations, dehydrations, reductions, and reductive dehalogenations Limosilactobacillus reuteri CRL1098 adenosylcobalamin + triphosphate
-
?
cob(I)alamin + ATP adenosylation of the corrinoid ring of cob(I)alamin, active site structure, ATP binding motif at the protein N terminus Limosilactobacillus reuteri CRL1098 adenosylcobalamin + triphosphate
-
?

Synonyms

Synonyms Comment Organism
PduO
-
Limosilactobacillus reuteri
PduO-type ATP:Co(I)rrinoid adenosyltransferase
-
Limosilactobacillus reuteri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Limosilactobacillus reuteri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.025
-
ATP
-
Limosilactobacillus reuteri
0.025
-
cob(I)alamin
-
Limosilactobacillus reuteri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Limosilactobacillus reuteri

Cofactor

Cofactor Comment Organism Structure
ATP ATP binding motif at the protein N terminus Limosilactobacillus reuteri