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Literature summary for 2.5.1.17 extracted from

  • Beck, W.S.
    Ribosome-associated vitamin B12s adenosylating enzyme of Lactobacillus leichmannii (1980), Methods Enzymol., 67, 41-56.
    View publication on PubMed

General Stability

General Stability Organism
2-mercaptoethanol stabilizes during purification Clostridium tetanomorphum
2-mercaptoethanol stabilizes during purification Lactobacillus leichmannii
repeated freeze-thawing inactivates Clostridium tetanomorphum
repeated freeze-thawing inactivates Lactobacillus leichmannii

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Lactobacillus leichmannii
Cd2+
-
Lactobacillus leichmannii
diphosphate
-
Clostridium tetanomorphum
diphosphate
-
Lactobacillus leichmannii
Mn2+ above 10 mM Lactobacillus leichmannii
tripolyphosphate
-
Clostridium tetanomorphum
tripolyphosphate
-
Lactobacillus leichmannii
Zn2+
-
Lactobacillus leichmannii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
ATP pH 8.0, 37°C Lactobacillus leichmannii

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information subcellular distribution Lactobacillus leichmannii
-
-
ribosome
-
Lactobacillus delbrueckii 5840
-
ribosome
-
Lactobacillus leichmannii 5840
-
soluble
-
Clostridium tetanomorphum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+ activation Lactobacillus leichmannii
K+
-
Clostridium tetanomorphum
K+ activation Lactobacillus leichmannii
K+ may be acting upon a step in the reduction of hydroxocobamide Propionibacterium freudenreichii subsp. shermanii
Mg2+
-
Propionibacterium freudenreichii subsp. shermanii
Mg2+ activation, less effective than Mn2+ Clostridium tetanomorphum
Mg2+ activation, less effective than Mn2+ Lactobacillus leichmannii
Mn2+
-
Clostridium tetanomorphum
Mn2+
-
Lactobacillus leichmannii
Na+ activation Lactobacillus leichmannii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + cob(I)alamin Propionibacterium freudenreichii subsp. shermanii
-
triphosphate + coenzyme B12
-
?
ATP + cob(I)alamin Lactobacillus delbrueckii
-
triphosphate + coenzyme B12
-
?
ATP + cob(I)alamin Clostridium tetanomorphum
-
triphosphate + coenzyme B12
-
?
ATP + cob(I)alamin Lactobacillus leichmannii
-
triphosphate + coenzyme B12
-
?

Organism

Organism UniProt Comment Textmining
Clostridium tetanomorphum
-
-
-
Lactobacillus delbrueckii
-
-
-
Lactobacillus leichmannii
-
-
-
Propionibacterium freudenreichii subsp. shermanii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification of the enzyme is achieved from ribosomal fraction by salt extraction and ammonium sulfate fractionation, followed by two chromatographic steps Lactobacillus leichmannii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000125
-
activity measured using washed ribosomes Lactobacillus leichmannii
0.000125
-
pH 8.0, 37°C Lactobacillus leichmannii

Storage Stability

Storage Stability Organism
-20°C, more than 50% loss of activity within 1 week, with 50% glycerol only 20% loss of activity Lactobacillus leichmannii
2-mercaptoethanol stabilizes during storage Clostridium tetanomorphum
2-mercaptoethanol stabilizes during storage Lactobacillus leichmannii
DTT stabilizes during storage Lactobacillus leichmannii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + cob(I)alamin
-
Propionibacterium freudenreichii subsp. shermanii triphosphate + coenzyme B12
-
?
ATP + cob(I)alamin
-
Lactobacillus delbrueckii triphosphate + coenzyme B12
-
?
ATP + cob(I)alamin
-
Clostridium tetanomorphum triphosphate + coenzyme B12
-
?
ATP + cob(I)alamin
-
Lactobacillus leichmannii triphosphate + coenzyme B12
-
?
ATP + cob(I)alamin
-
Propionibacterium freudenreichii subsp. shermanii triphosphate + coenzyme B12 adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 ?
ATP + cob(I)alamin
-
Lactobacillus delbrueckii triphosphate + coenzyme B12 adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 ?
ATP + cob(I)alamin
-
Clostridium tetanomorphum triphosphate + coenzyme B12 adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 ?
ATP + cob(I)alamin
-
Lactobacillus leichmannii triphosphate + coenzyme B12 adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 ?
additional information
-
Propionibacterium freudenreichii subsp. shermanii ?
-
?
additional information
-
Clostridium tetanomorphum ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Clostridium tetanomorphum
37
-
assay at Lactobacillus leichmannii