General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes during purification | Clostridium tetanomorphum |
2-mercaptoethanol stabilizes during purification | Lactobacillus leichmannii |
repeated freeze-thawing inactivates | Clostridium tetanomorphum |
repeated freeze-thawing inactivates | Lactobacillus leichmannii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Lactobacillus leichmannii | |
Cd2+ | - |
Lactobacillus leichmannii | |
diphosphate | - |
Clostridium tetanomorphum | |
diphosphate | - |
Lactobacillus leichmannii | |
Mn2+ | above 10 mM | Lactobacillus leichmannii | |
tripolyphosphate | - |
Clostridium tetanomorphum | |
tripolyphosphate | - |
Lactobacillus leichmannii | |
Zn2+ | - |
Lactobacillus leichmannii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
ATP | pH 8.0, 37°C | Lactobacillus leichmannii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | subcellular distribution | Lactobacillus leichmannii | - |
- |
ribosome | - |
Lactobacillus delbrueckii | 5840 | - |
ribosome | - |
Lactobacillus leichmannii | 5840 | - |
soluble | - |
Clostridium tetanomorphum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cs+ | activation | Lactobacillus leichmannii | |
K+ | - |
Clostridium tetanomorphum | |
K+ | activation | Lactobacillus leichmannii | |
K+ | may be acting upon a step in the reduction of hydroxocobamide | Propionibacterium freudenreichii subsp. shermanii | |
Mg2+ | - |
Propionibacterium freudenreichii subsp. shermanii | |
Mg2+ | activation, less effective than Mn2+ | Clostridium tetanomorphum | |
Mg2+ | activation, less effective than Mn2+ | Lactobacillus leichmannii | |
Mn2+ | - |
Clostridium tetanomorphum | |
Mn2+ | - |
Lactobacillus leichmannii | |
Na+ | activation | Lactobacillus leichmannii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cob(I)alamin | Propionibacterium freudenreichii subsp. shermanii | - |
triphosphate + coenzyme B12 | - |
? | |
ATP + cob(I)alamin | Lactobacillus delbrueckii | - |
triphosphate + coenzyme B12 | - |
? | |
ATP + cob(I)alamin | Clostridium tetanomorphum | - |
triphosphate + coenzyme B12 | - |
? | |
ATP + cob(I)alamin | Lactobacillus leichmannii | - |
triphosphate + coenzyme B12 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium tetanomorphum | - |
- |
- |
Lactobacillus delbrueckii | - |
- |
- |
Lactobacillus leichmannii | - |
- |
- |
Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification of the enzyme is achieved from ribosomal fraction by salt extraction and ammonium sulfate fractionation, followed by two chromatographic steps | Lactobacillus leichmannii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000125 | - |
activity measured using washed ribosomes | Lactobacillus leichmannii |
0.000125 | - |
pH 8.0, 37°C | Lactobacillus leichmannii |
Storage Stability | Organism |
---|---|
-20°C, more than 50% loss of activity within 1 week, with 50% glycerol only 20% loss of activity | Lactobacillus leichmannii |
2-mercaptoethanol stabilizes during storage | Clostridium tetanomorphum |
2-mercaptoethanol stabilizes during storage | Lactobacillus leichmannii |
DTT stabilizes during storage | Lactobacillus leichmannii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cob(I)alamin | - |
Propionibacterium freudenreichii subsp. shermanii | triphosphate + coenzyme B12 | - |
? | |
ATP + cob(I)alamin | - |
Lactobacillus delbrueckii | triphosphate + coenzyme B12 | - |
? | |
ATP + cob(I)alamin | - |
Clostridium tetanomorphum | triphosphate + coenzyme B12 | - |
? | |
ATP + cob(I)alamin | - |
Lactobacillus leichmannii | triphosphate + coenzyme B12 | - |
? | |
ATP + cob(I)alamin | - |
Propionibacterium freudenreichii subsp. shermanii | triphosphate + coenzyme B12 | adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 | ? | |
ATP + cob(I)alamin | - |
Lactobacillus delbrueckii | triphosphate + coenzyme B12 | adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 | ? | |
ATP + cob(I)alamin | - |
Clostridium tetanomorphum | triphosphate + coenzyme B12 | adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 | ? | |
ATP + cob(I)alamin | - |
Lactobacillus leichmannii | triphosphate + coenzyme B12 | adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 | ? | |
additional information | - |
Propionibacterium freudenreichii subsp. shermanii | ? | - |
? | |
additional information | - |
Clostridium tetanomorphum | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Clostridium tetanomorphum |
37 | - |
assay at | Lactobacillus leichmannii |