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Literature summary for 2.5.1.16 extracted from

  • Korolev, S.; Ikeguchi, Y.; Skarina T.; Beasley, S.; Arrowsmith, C.; Edwards, A.; Joachimiak, A.; Pegg, A.E.; Savchenko, A.
    The crystal structure of spermidine synthase with a multisubstrate adduct inhibitor (2002), Nat. Struct. Biol., 9, 27-31.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.16

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion, multiwavelength anomalous diffraction from selenomethionine containing crystals Thermotoga maritima

General Stability

General Stability Organism
tetramerization stabilizes Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
S-Adenosyl-1,8-diamino-3-thiooctane IC50 of 0.0002 mM Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 1,4-diaminobutane pH 7.5, 37ºC Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
131000
-
 gel filtration Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane Thermotoga maritima
-
 5'-methylthioadenosine + spermidine
-
 ?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,3-diaminopropane weak substrate Thermotoga maritima ?
-
 ?
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Thermotoga maritima 5'-methylthioadenosine + spermidine
-
 ?
S-adenosyl-(5')-3-methylthio-1-propylamine + spermidine weak substrate Thermotoga maritima 5'-methylthioadenosine + spermine
-
 ?
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Thermotoga maritima 5'-methylthioadenosine + spermidine
-
 ?
S-adenosyl-3-methylthio-1-propylamine + 1,5-diaminopentane weak substrate Thermotoga maritima 5'-methylthioadenosine + N-(3-aminopropyl)-1,5-diaminopentane
-
 ?

Subunits

Subunits Comment Organism
tetramer HPLC size-exclusion chromatography Thermotoga maritima

Synonyms

Synonyms Comment Organism
PAPT
-
-

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
 more active than at 37ºC Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Thermotoga maritima

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0002
-
 IC50 of 0.0002 mM Thermotoga maritima S-Adenosyl-1,8-diamino-3-thiooctane